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EP1230354: PERMANENT AMNIOCYTE CELL LINE, THE PRODUCTION THEREOF AND ITS USE FOR PRODUCING GENE TRANSFER VECTORS

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Filing Information

Inventor(s) KOCHANEK STEFAN · SCHIEDNER GUDRUN ·
Assignee(s) KOCHANEK STEFAN ·
Attorney/Agent(s) Dehmel, Albrecht, Dr. ·
Application Number EP00979539
Filing date 11/07/2000
Publication date 08/14/2002
U.S. Classifications --
International Classifications C12N 5/10  · C12N 7/00  · C12N 15/09  · C12N 15/861  ·
Kind CodeA2
PCT Application Number EP2000010992 - 11/07/2000
PCT Publication Number WO2001036615 - 05/25/2001
22 Claims, 7 Drawings


Abstract

The current document has no english abstract.

Independent Claims | See all claims (66)

  1. A permanent amniocytic cell line comprising at least one nucleic acid which brings about expression of the gene products of the adenovirus E1A and E1B regions.
  2. A process for producing a permanent amniocytic cell line, characterized by the transfection of amniocytes with at least one nucleic acid, which brings about the expression of the adenoviral gene products of the E1A region and E1B region.
  3. The permanent amniocytic cell line N52.E6 (DSM ACC2416).
  4. Use of amniocytes for producing adenovirus-transformed permanent amniocytic cell lines.
  5. Use of the adenoviral gene products of the E1A and E1B regions for producing permanent amniocytic cell lines.
  6. Use of a permanent amniocytic cell line for producing gene transfer vectors and/or adenovirus mutants.
  7. A process for producing gene transfer vectors and/or adenovirus mutants, wherein a permanent amniocytic cell line is used.

References Cited

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
WO199700326

Other Publications

SCHIEDNER GUDRUN ET AL: "Efficient transformation of primary human amniocytes by E1 functions of Ad5: Generation of new cell lines for adenoviral vector production." HUMAN GENE THERAPY, Bd. 11, Nr. 15, 10. Oktober 2000 (2000-10-10), Seiten 2105-2116, XP000974022 ISSN: 1043-0342
PLOUZEK C A ET AL: "ISOLATION AND CHARACTERIZATION OF A HUMAN AMNION EPITHELIAL CELL LINE THAT EXPRESSES THE PREGNANCY-SPECIFIC BETA-1 GLYCOPROTEIN GENE" ENDOCRINOLOGY, Bd. 129, Nr. 2, 1991, Seiten 950-958, XP000974066 ISSN: 0013-7227

Referenced By

Document NumberAssigneeInventorsIssue/Pub Date
US8574595 --
US8460920 --