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US20090226879: Protein-Free Gamete and Embryo Handling and Culture Media Products

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Filing Information

Inventor(s) Jaffar Ali bin M. Abdullah ·
Assignee(s) CELLCURA, INC. ·
Correspondent MCDONNELL BOEHNEN HULBERT & BERGHOFF LLP ·
Application Number US12340475
Filing date 12/19/2008
Publication date 09/10/2009
Predicted expiration date 12/19/2028
U.S. Classifications 435/2  · 435/366  · 435/404  ·
International Classifications A01N102  · C12N508  ·
Kind CodeA1
50 Claims, No Drawings


Abstract

This invention discloses a substantially protein-free cell culture solution for assisted reproductive technologies and methods of use thereof.

Independent Claims | See all claims (50)

  1. 1. A substantially protein-free cell culture medium for human reproductive cells, suitable for use throughout in vitro treatment of human reproductive cells to be used in in vitro fertilization and other assisted reproduction technologies, comprising mineral salts, amino acids, antioxidants, vitamins, nutrients, antibiotics, D-mannitol, and methylcellulose that has a molecular weight of 14,000 Daltons.
  2. 45. A substantially protein-free cell culture medium for human reproductive cells, suitable for use throughout in vitro treatment of human reproductive cells to be used in in vitro fertilization and other assisted reproduction technologies, comprising 0.1 g/L (0.0071 mM) methylcellulose having a molecular weight of 14,000 Daltons; 0.5 mM L-arginine; 0.01 mM L-cystine.2HCl; 0.02 mM L-histidine, 0.04 mM L-isoleucine; 0.04 mM L-leucine; 0.05 mM L-lysine.HCl; 0.01 mM L-methionine; 0.02 mM L-phenylalanine; 0.04 mM L-threonine; 0.005 mM L-tryptophan; 0.02 mM L-tyrosine.2Na2H2O; 0.04 mM L-valine; 0.5 mM L-alanine; 20 mM for L-taurine; 0.5 mM glutamic acid (0.39 mM); 20 mM L-glutamine or 0.25 mM L-Glycine; 3.1 mM (0.235 g/L) calcium chloride; 0.72 mM (0.087 g/L) magnesium sulfate; 4.8 mM (0.355 g/L) potassium chloride; 0.11 M (6.171 g/L) sodium chloride; 0.88 mM (0.108 g/L) for sodium dihydrogen phosphate; 0.004 mM (0.0005 g/L) choline chloride; 0.0024 mM (0.0006 g/L) D-biotin; 0.0067 mM (0.0012 g/L) myoinositol; 0.005 mM (0.0006 g/L) niacinamide; 0.0025 mM (0.0005 g/L) D-pantothenic acid; 0.003 (0.0006 g/L) pyridoxine HCl; 0.00016 mM (0.00006 g/L) riboflavin; 0.0018 mM (0.0005 g/L) thiamine HCl; 0.0014 mM (0.006 g/L) folic acid; 616 pM (800 ng/L) vitamin B12; 0.010 mM (0.004 g/L) vitamin E; 4.3 mM (0.78 g/L) D-glucose; 0.3 mM (0.02975 g/L) sodium pyruvate; 5.1 mM (0.92 g/L) fructose; 10 mM (1.13 g/L) sodium lactate; 0.3 mM (0.092 g/L) glutathione; 0.1 mM (0.0418 g/L) EDTA; 0.031 mM (0.011 g/L) phenol red; 2.8 micromolar D-mannitol; 26.2 mM (2.2 g/L) sodium bicarbonate; 75 mg/L Penicillin G; and 1.5 mg/L gentamycin sulfate.

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