Filing Information

Inventor(s)	Sergey Shevkoplyas · Tatsuro Yoshida ·
Assignee(s)	The Trustees of Boston University ·  View assignee updates
Correspondent	Paul D. Greeley;Ohlandt, Greeley, Ruggiero & Perle, L.L.P. ·
Application Number	US12386108
Filing date	04/14/2009
Publication date	10/29/2009
Predicted expiration date	03/01/2023
U.S. Classifications	435/287.1  · 382/134  · 702/19  · 707/104.1  · 435/29  ·
International Classifications	C12M300  · C12Q102  · G01N3348  · G06F1730  · G06F1900  · G06K900  ·
Kind Code	A1

Patent Family

Abstract

A system for assessing the microvascular fitness of a sample of stored red blood cells. The system has a network device having at least one network unit. The network unit has a single inlet and a single outlet for the sample and a plurality of microchannels. The plurality of microchannels receive the sample from the single inlet and drain the sample into the single outlet. The network unit includes an aspiration pressure means for providing movement of liquid sample through the at least one network unit. The system further includes an analysis unit that receives the network device therein. The analysis unit includes a sensor for capturing measurements related to the sample and a processor capable of comparing the captured measurements to measurements stored in a database of healthy red blood cells to determine the microvascular fitness of the stored red blood cells.

Independent Claims | See all claims (37)

1. 1. A system for assessing the microvascular fitness of a sample of stored red blood cells comprising: a network device comprising: at least one network unit disposed in said device; said at least one network unit comprising of a single inlet and a single outlet for the sample; a plurality of microchannels; wherein said plurality of microchannels receives the sample from said single inlet and drains the sample into said single outlet; an aspiration pressure means for providing movement of liquid sample through said at least one network unit; and an analysis device that receives said network device therein, said analysis device comprising: a sensor for capturing measurements related to said sample; and a processor capable of comparing said captured measurements to measurements stored in a database of healthy red blood cells to determine the microvascular fitness of the stored red blood cells.
2. 18. A method for assessing the microvascular fitness of a sample of stored red blood cells comprising: obtaining and storing measurements from a plurality of samples of healthy red blood cells; flowing a sample of stored red blood cells through a network device and sensing measurements related to the stored sample; and comparing measurements obtained from said plurality of samples of healthy red blood cells to measurements derived from said stored red blood cells to determine the microvascular fitness of said stored red blood cells.
3. 30. A microchannel network device comprising: at least one network unit comprising of a single inlet and a single outlet for the sample, said at least one network device also comprising a plurality of microchannels; wherein said plurality of microchannels receives the sample from said single inlet and drains the sample into said single outlet; an aspiration pressure means for providing movement of liquid sample through said at least one network device; and a substrate disposed beneath said at least one network unit; wherein each of said plurality of microchannels is either i) a parent microchannel that branches into two daughter microchannels at an angle of from approximately 20° to 80°, or ii) a convergence of two daughter microchannels into a convergence channel at an angle of from approximately 20° to 80°.