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US4082613: Process for the production of insulin by genetically transformed fungal cells
6 Claims, No Drawings
A process for the production of insulin using fungal cells. Human insulin producing cells are extracted to obtain the functional genome (the genetic material which determines the capacity of the cells to produce insulin) which is used to transform fungal cells, making them capable of producing insulin. The insulin has been extracted and identified by radioimmune assay and by bioassay. The same process can also be applied to produce animal insulins using specific specie transforming functional insulinogenic genomes. A process for serial secondary culture of insulin producing cells is disclosed. The significance of the invention is related to the great need to produce human insulin in large amounts. Human insulin has distinct biological and clinical advantages when compared with commercially available animal insulins now used for the treatment of diabetes mellitus in man.
- 1. A process for the production of insulin by genetically transformed fungal cells which comprises:A. selectively growing insulin producing beta-epithelioid cells from a pancreas and serially sub-culturing the cells under aerated cell growth conditions in a nutrient amino acid-rich medium,B. inoculating the rapid growth fungus Pseudosaccharomycete TC-1176 with genomic material extracted from the sub-culture cells and incubating in the presence of an anti-fungal membrane permeability agent to incorporate the functional genome into the fungal cell structure,C. incubating the resultant bio-transformed fungal cells in a carbohydrate-nitrogen-rich medium under cell growth conditions, andD. separating the fungal cells from the media and extracting the insulin from the cells and supernatant media.
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|vanek et al., Genetics of Industrial Microorganisms, vol. II, p. 38, Elsevier Pub. Co., 1973.|
|Willmer, Cells and Tissues in Culture, vol. 2, p. 674, Academic Press, 1965.|