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US4225671: Process for the in-vitro biosynthesis of hormones, especially insulin

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Filing Information

Inventor(s) Herwig Puchinger · Ulrich Mueller · Manfred Sernetz ·
Assignee(s) Battelle-Institut e.V. ·
Attorney/Agent(s) Fisher, Christen & Sabol ·
Primary Examiner Alvin E. Tanenholtz ·
Application Number US5914138
Filing date 06/09/1978
Issue date 09/30/1980
Predicted expiration date 06/09/1998
U.S. Classifications 435/71  · 435/240  ·
International Classifications C12P 2104  ·
Kind CodeA
International Classifications 1951;127;142;1.7;1.8 ·
Foreign Priority DE2726313 - 06/10/1977 ·
12 Claims, No Drawings


Abstract

Process for the in-vitro biosynthesis of hormones, especially of insulin, by in-vitro conservation or in-vitro propagation of cells from organs and tissues of animal or human origin under suitable cell growth and cell conservation conditions and isolation of the substance formed in a known manner from the culture medium or by processing of the cells. The hormone-producing cells are propagated or conserved in one or more cell culture spaces separated by semipermeable flat membranes of at least one culture medium space.

Independent Claims | See all claims (12)

  1. 1. Process for the in-vitro biosynthesis of a hormone comprising the steps of (a) introducing (i) cells from organs or tissues of animal or human origin into one or more cell culture spaces and (ii) culture medium separately into one or more culture medium spaces, each cell culture space having a flat top and flat bottom, each culture medium space having a flat top and a flat bottom, when more than one culture medium space and more than one cell culture space are used, the culture medium spaces are in alternating relationship to the cell culture spaces, the culture medium spaces are adjacent to the cell culture spaces, and a culture medium space is located on top of each cell culture space, when one culture medium space and one cell culture space are present, the culture medium space is located adjacent to and on top of the cell culture space, and each culture medium space is separated from each adjacent cell culture space or spaces by a semi-permeable flat membrane which is permeable to liquid and is impermeable to cells, (b) propogating or cultivating in vitro the cells under suitable cell growth or cell cultivation conditions, respectively, whereby the cells in vitro biosynthesize the hormone, and (c) isolating the resultant hormone from the culture medium and/or by processing the cells after removing the cells from the cell culture space.

References Cited

U.S. Patent Documents

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US3948732 Instrumentation Laboratory, Inc. Haddad et al. Apr 1976
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