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US4601978: Mammalian metallothionein promoter system

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Filing Information

Inventor(s) Michael Karin ·
Assignee(s) The Regents of the University of California ·
Attorney/Agent(s) Townsend and Townsend ·
Primary Examiner Thomas G. Wiseman ·
Assistant Examiner James Martinell ·
Application Number US6444134
Filing date 11/24/1982
Issue date 07/22/1986
Predicted expiration date 07/22/2003
U.S. Classifications 435/68  · 435/70  ·
International Classifications C12N 120  ·
Kind CodeA
International Classifications 43568;70;91;172;253;317;240;241;172.3 ·
16 Claims, No Drawings


Abstract

A cloning vector comprises a replication system from a bovine papilloma virus, a regulatory system including the promoter and terminator from a human metallothionein II gene, and a prokaryotic replication system. The vector is useful for transforming mammalian cells where it is maintained as an automonously replicating episome. Transcription may be regulated by the presence of heavy metals and/or glucocorticoid hormones in the growth media.

Independent Claims | See all claims (16)

  1. 1. A DNA sequence of less than 500 base pairs, said DNA sequence comprising the human MT-II transcriptional regulatory system further comprising the transcription initiation sequence.
  2. 5. A DNA construct capable of regulated expression of an inserted gene in a mammalian host, said construct comprising an extrachromosomal replication system recognized by a mammalian host, which replication system has been joined to a human MT-II gene inducible regulatory system in vitro, said construct having DNA sequence(s) defining at least one restriction site within the transcriptional control of the regulatory region for insertion of the gene.
  3. 10. A DNA construct useful for expression of a structural gene to produce a polypeptide in a mammalian host, said vector comprising (a) a replicon from bovine papilloma virus and (b) a regulatory system from a human MT-II gene comprising a promoter and a terminator and having at least one restriction site downstream from the promoter in the direction of transcription.

References Cited

The current document has no citations.

Other Publications

Karin et al., Nucleic Acids Res. 10, 3165 (1982).
Durnam et al., Proc. Natl. Acad. Sci. USA 77, 6511 (1980).
Pulido et al., Biochemistry 5, 1768 (1966).
Brinster et al., Nature 296, 39 (1982).
Hager et al., Nature 291, 340 (1981).
Karin, Diss. Abstr. 40(9), 4084 (1980).
Douglas R. Lowy, et al,. Nature (1980) 287:72-74.
Ming-Fan Law et al., Proc. Natl. Acad. Sci. USA (1981) 78:2727-2731.
Sarver et al., Preprint of Mol. Cell Biol. (1981).

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