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US4937196: Membrane bioreactor system

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Filing Information

Inventor(s) Wolfgang J. Wrasidlo · Frieder K. Hofmann · Dirk M. deWinter ·
Assignee(s) Brunswick Corporation ·
Attorney/Agent(s) Waldron & Associates ·
Primary Examiner Larry Jones ·
Application Number US7395655
Filing date 08/18/1989
Issue date 06/26/1990
Predicted expiration date 08/18/2009
U.S. Classifications 435/313  · 435/284  ·
International Classifications C12M 104  ·
Kind CodeA
International Classifications 435313;310;284;287;240.242;286 ·
Related U.S. Application DataThis application is a continuation of applicant's copending application, Ser. No. 07/068,203, filed Jun. 30, 1987, now abandoned benefit of which is hereby claimed under 35 U.S.C. 120.
25 Claims, No Drawings


Abstract

A bioreactor is disclosed in which cells are confined to a compartment formed by porous hydrophyllic sheet membranes through which a nutrient solution diffuses in and exocellular products and metabolic waste diffuse out. Adjacent gas compartments allow the flow of free oxygen into the cell compartment. Each cell compartment is configured to place cells within about 100-200 micrometers of the oxygen transport membrane.

Independent Claims | See all claims (25)

  1. 1. A cell reactor comprising:A. at least one layered cell reactor compartment for confining and retaining living cells and nutrient solution to produce exocellular products,B. at least one associated nutrient compartment for feeding liquid cell nutrients to each said cell reactor compartment and for the removal of exocellular products and metabolic wastes from said cell reactor compartment,C. each said cell reactor compartment and said associated nutrient compartment having a common boundary formed by a porous hydrophyllic sheet membrane having a pore size adapted to permit flow therethrough by diffusion of nutrient, exocellular products and metabolic wastes, and preventing passage therethrough of cells,D. at least one associated gas compartment for the supply of free oxygen into solution in nutrient solution in each said cell reactor compartment,E. each said cell reactor compartment and said associated gas compartment having a common boundary formed by an oxygen transport membrane adapted to permit flow therethrough of free oxygen and adapted to prevent flow of cells, nutrient, exocellular products and metabolic wastes into said gas compartment,F. each said cell reactor compartment and said associated oxygen transport membrane being disposed so that substantially all cells within said cell reactor compartment are within effective oxygen diffusion distance of up to about 100 micrometers to about 200 micrometers of said oxygen transport membrane, said oxygen diffusion distance being effective to maintain cell viability and cell densities greater than about 5.times.108 cells per milliliter by transport of dissolved oxygen from said oxygen transport membrane to said cells solely by diffusion; andG. each said cell reactor compartment layer is separated from each adjacent cell reactor chamber layer by either (i) a nutrient transport compartment or (ii) a gas compartment on one side and a nutrient transport compartment on an opposite side.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US3489647 DOW CORNING Kolobom Jan 1970
US3734851 MATSUMURA K Matsumura May 1973
US3809613 RESEARCH CORP Vieth et al. May 1974
US3948732 Instrumentation Laboratory, Inc. Haddad et al. Apr 1976
US4087327 Monsanto Company Feder et al. May 1978
US4201845 Monsanto Company Feder et al. May 1980
US4220725 United States of America Knazek et al. Sep 1980
US4225671 Battelle-Institut e.V. Puchinger et al. Sep 1980
US4241187 United States of America White Dec 1980
US4391912 Asahi Kasei Kogyo Kabushiki Kaisha Yoshida et al. Jul 1983
US4537860 Monsanto Company Tolbert et al. Aug 1985
US4661455 Dorr-Oliver Incorporated Hubbard Apr 1987
US4661458 Cell Environmental Systems, Ltd. Berry et al. Apr 1987
US4689301 Commissariat a l'Energie Atomique Adet et al. Aug 1987
US4693983 National Research Development Corporation Davies et al. Sep 1987
US4720462 ROSENSON ROBERT Rosenson Jan 1988
US4804628 Endotronics, Inc. Cracaner et al. Feb 1989

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
JP39875Sep 1981
JP256375Dec 1985
GB2178447YEDA RES & DEVFeb 1987

Other Publications

Biotechnology and Bioengineering, vol. XXIII, pp. 79-95 (1981), (K. Ku et al.), "Development of a Hollow-Fiber System for Large-Scale Culture of Mammalian Cells", see pp. 83-94.

Referenced By

Document NumberAssigneeInventorsIssue/Pub Date
DE4208429 MAERKL HERBERT MAERKL HERBERT PROF DR ING Sep 1993
US6410307 Acordis Industrial Fibers GmbH Herma Glockner et al. Jun 2002
US6916652 Kinetic Biosystems, Inc. Peter J. Petrecca et al. Jul 2005
US7594663 ZymeQuest, Inc. Glen Jorgensen et al. Sep 2009
US6562616 The General Hospital Corporation Mehmet Toner et al. May 2003
US6773458 Baxter International Inc. James H. Brauker et al. Aug 2004
US6855542 Organogenesis Inc. Paul A. DiMilla et al. Feb 2005
US6589790 Bio Merieux Bruno Colin et al. Jul 2003
US6660509 Kinetic Biosystems, Inc. Heath H. Herman et al. Dec 2003
US6759245 The General Hospital Corporation Mehmet Toner et al. Jul 2004
US6916630 Samir S Sofer Jul 2005
US7875176 Porous Media Corporation Philip M. Rolchigo et al. Jan 2011
US7695624 OTV SA Martin Brockmann et al. Apr 2010
US7745201 Algenion GmbH & Co. KG Michael Melkonian et al. Jun 2010
US7790444 Universita' Degli Studi di Pisa Arti Ahluwalia et al. Sep 2010
US6703217 Kinetic BioSystems, Inc. Heath H. Herman et al. Mar 2004
US7951555 Australian Nuclear Science and Technology Organisation Anthony Patrick Andrew Taylor et al. May 2011
US8158426 Wilson Wolf Manufacturing Corporation John R. Wilson et al. Apr 2012
US8158427 Wilson Wolf Manufacturing Corporation John R. Wilson et al. Apr 2012
EP1132460 Sefar AG Mayer, Christine, Mag. rer. nat. et al. Sep 2001
EP1765498 UNIVERSITA' DI PISA Arti AHLUWALIA et al. Mar 2010
EP1203075 THE GENERAL HOSPITAL CORPORATION Maury D. COSMAN et al. Sep 2007
EP1159444 Acordis Industrial Fibers GmbH Herma GLCKNER et al. May 2004
EP0626007 NORSKE MEIERIER Magne LYSBERG et al. Jun 1999
EP0769048 Wilson Wolf Corporation John Wilson et al. Apr 2005
EP0817834 Wilson Wolf Corporation Martin Wolf et al. Nov 2004
USD414272 bioMerieux Vitek, Inc. Raymond E. O'Bear et al. Sep 1999
US5713888 Baxter International, Inc. Steven Neuenfeldt et al. Feb 1998
US6306491 Gore Enterprise Holdings, Inc. Brian H. Kram et al. Oct 2001
US5693537 John R. Wilson et al. Dec 1997
US5453278 Baxter International Inc. Chan; Eddie K. M. et al. Sep 1995
US6133019 Kinetic Biosystems, Inc. Heath H. Herman Oct 2000
US5545223 Baxter International, Inc. Steven Neuenfeldt et al. Aug 1996
US5622819 Kinetic Biosystems, Inc. Heath H. Herman Apr 1997
US6214617 Kinetic Biosystems, Inc. Heath H. Herman Apr 2001
US5714384 John R. Wilson et al. Feb 1998
US5964804 Baxter International Inc. James H. Brauker et al. Oct 1999
US6156305 Baxter International Inc. James H. Brauker et al. Dec 2000
US5416022 Cellex Biosciences, Inc. Bruce P. Amiot May 1995
US5482859 Edmund Biller et al. Jan 1996
US5569462 Baxter International Inc. Laura A. Martinson et al. Oct 1996
US5733336 Baxter International, Inc. Steven Neuenfeldt et al. Mar 1998
US5821116 Kinetic Biosystems, Inc. Heath H. Herman Oct 1998
US5707869 Martin L. Wolf et al. Jan 1998
US5728581 Systemix, Inc. Richard Merrill Schwartz et al. Mar 1998
US5190878 Minuth Wilhelm Mar 1993
US8323499 Veolia Water Solutions & Technologies Support Hong W. Zhao et al. Dec 2012
US8415144 --
US8518692 --
US8697443 --
US8713850 --

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