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US5036007: Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation

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Filing Information

Inventor(s) Pramod K. Gupta · Gerald S. Pullman ·
Assignee(s) Weyerhaeuser Company ·
Primary Examiner Howard J. Locker ·
Assistant Examiner Gary Benzion ·
Application Number US7499151
Filing date 03/26/1990
Issue date 07/30/1991
Predicted expiration date 03/09/2009
U.S. Classifications 435/240.45  · 435/240.4  ·
International Classifications A01H 400  ·
Kind CodeA
International Classifications 435240.4;240.45;240.46;240.47;240.49;240.54 ·
Related U.S. Application DataThis application is a continuation-in-part of our earlier application Ser. No. 321,035, filed Mar. 9, 1989 now U.S. Pat. No. 4,957,866 and our pending Ser. No. 426,331, filed Oct. 23, 1989.
26 Claims, No Drawings


Abstract

The invention is a method for developing tissue culture induced coniferous somatic proembryos into well developed cotyledonary embryos. The method comprises a multistage culturing process in which early stage proembryos are cultured on a late stage proembryo medium comprising a significantly higher osmotic potential along with abscisic acid and an absorbent material to gradually reduce the level of available abscisic acid over time. Culturing from this point continues in an embryo development medium having a high osmotic potential in which the osmotic potential is preferably raised during embryo development to a final level of about 450 mM/kg. Through this process the vigor and morphology of the embryos is improved and the tendency to germinate prematurely is significantly reduced. After a period of several weeks in culture somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to the soil for further growth.

Independent Claims | See all claims (26)

  1. 1. A method suitable for further developing tissue culture induced coniferous plant species somatic proembryos into well developed cotyledonary embryos which comprises:transferring and further culturing the proembryos using a development medium having sufficient amounts of mineral and organic nutrient materials along with a sufficient initial amount of abscisic acid and an adsorbent material to gradually reduce the level of available abscisic acid over time, said medium also having sufficient osmoticants to raise the osmotic potential to at least about 350 mM/kg, so as to enable and promote the development and growth of robust cotyledonary embryos having a high potential for normal germination and plant development.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
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Other Publications

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Referenced By

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