Join
today

Boliven PRO is more than just patent search

  • Build and save lists using the powerful Lists feature
  • Analyze and download your search results
  • Share patent search results with your clients

Patents »

US5036007: Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation

Share

Filing Information

Inventor(s) Pramod K. Gupta · Gerald S. Pullman ·
Assignee(s) Weyerhaeuser Company ·
Primary Examiner Howard J. Locker ·
Assistant Examiner Gary Benzion ·
Application Number US7499151
Filing date 03/26/1990
Issue date 07/30/1991
Predicted expiration date 03/09/2009
U.S. Classifications 435/240.45  · 435/240.4  ·
International Classifications A01H 400  ·
Kind CodeA
International Classifications 435240.4;240.45;240.46;240.47;240.49;240.54 ·
Related U.S. Application DataThis application is a continuation-in-part of our earlier application Ser. No. 321,035, filed Mar. 9, 1989 now U.S. Pat. No. 4,957,866 and our pending Ser. No. 426,331, filed Oct. 23, 1989.
26 Claims, No Drawings


Abstract

The invention is a method for developing tissue culture induced coniferous somatic proembryos into well developed cotyledonary embryos. The method comprises a multistage culturing process in which early stage proembryos are cultured on a late stage proembryo medium comprising a significantly higher osmotic potential along with abscisic acid and an absorbent material to gradually reduce the level of available abscisic acid over time. Culturing from this point continues in an embryo development medium having a high osmotic potential in which the osmotic potential is preferably raised during embryo development to a final level of about 450 mM/kg. Through this process the vigor and morphology of the embryos is improved and the tendency to germinate prematurely is significantly reduced. After a period of several weeks in culture somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to the soil for further growth.

Independent Claims | See all claims (26)

  1. 1. A method suitable for further developing tissue culture induced coniferous plant species somatic proembryos into well developed cotyledonary embryos which comprises:transferring and further culturing the proembryos using a development medium having sufficient amounts of mineral and organic nutrient materials along with a sufficient initial amount of abscisic acid and an adsorbent material to gradually reduce the level of available abscisic acid over time, said medium also having sufficient osmoticants to raise the osmotic potential to at least about 350 mM/kg, so as to enable and promote the development and growth of robust cotyledonary embryos having a high potential for normal germination and plant development.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US4217730 Weyerhaeuser Company El-Nil Aug 1980

Other Publications

Hakman et al., (1985), J. Plant Physiology, vol. 121, pp. 149-158.
Von Arnold et al., (1987), J. Plant Physiology, vol. 128, pp. 233-244.
Panott et al., (1988), In vitro Cellular & Developmental Biology, vol. 24, #8, pp. 817-820.
Fridborg et al., (1975), Physiol Plant, vol. 34, pp. 306-308.
Bu et al., (1988) ACTA Phytophysiol Sin 14(4), pp. 401-405.
Becwar, M. R. and R. P. Feirer, 1989, Factors Regulating Loblolly Pine Pinus taeda L.) Somatic Embryo Development, Institute of Paper Chemistry Report, Southern Forest Tree Improvement Conference, Raleigh, N.C., Jun. 1989.
Becwar, M. R., T. L. Noland, and S. R. Wann, 1987, A Method for Quantification of the Level of Somatic Embryogenesis Among Norway Spruce Callus Lines, Plant Cell Reports 6: 35-38.
Becwar, M. R., S. R. Wann, and R. Nagmani, 1988, A Survey of Initiation Frequency of Embryogenic Callus Among Ten Families of Pinus taeda (loblolly pine), Abstracts, 4th International Conifer Tissue Culture Work Group, Aug. 8-12, 1988, Saskatoon, Saskatchewan, Canada.
Boulay, M. P., P. K. Gupta, P. Krogstrup, and D. J. Durzan, 1988, Development of Somatic Embryos from Cell Suspension Cultures of Norway Spruce (Picea abies Karst.), Plant Cell Reports 7: 134-137.
Bourgkard, F. and J. M. Favre, 1988, Somatic Embryos from Callus of Sequoia Sempervirens, Plant Cell Reports 7: 445-448.
Buchheim, Julie A., Susan M. Colburn, and Jerome P. Ranch, 1989, Maturation of Soybean Somatic Embryos and the Transition to Plantlet Growth. Plant Physiology 89: 768-775.
Durzan, D. J. and P. K. Gupta, 1987, Somatic Embryogenesis and Polyembryogenesis in Douglas-Fir Cell Suspension Cultures, Plant Science 52: 229-235.
Finer, John J., Howard B. Kriebel, and Michael R. Becwar, 1989, Initiation of Embryogenic Callus and Suspension Cultures of Eastern White Pine (Pinus strobus L.), Plant Cell Reports 8: 203-206.
Gupta, Pramod K. and Don J. Durzan, 1985, Shoot Multiplication from Mature Trees of Douglas-Fir (Pseudotsuga menziesii) and Sugar Pine (Pinus lambertiana), Plant Cell Reports 4: 177-179.
1986a, Somatic Polyembryogenesis from Callus of Mature Sugar Pine Embryos, Bio/Technology 4: 643-645.
Gupta, Pramod and Don J. Durzan (cont.), 1986b, Plantlet Regeneration via Somatic Embryogenesis from Subcultured Callus of Mature Embryos of Picea abies (Norway Spruce), In Vitro Cellular and Developmental Biology 22: 685-688.
1987, Biotechnology of Somatic Polyembryogenesis and Plantlet Regeneration in Loblolly Pine, Bio/Technology 5: 147-151.
1988, Somatic Embryogenesis and Plant Regeneration from Suspension Cultures of Picea glauca (White Spruce), Physiologia Plantarum 72: 579-587.
Hakman, Inger, L. C. Fowke, Sara von Arnold, and Tage Eriksson, 1985, The Development of Somatic Embryos in Tissue Cultures Initiated from Immature Embryos of Picea abies (Norway Spruce), Plant Science 38: 33-35.
Johansson, Lars, 1983, Effects of Activated Charcoal in Anther Cultures, Physiologia Plantarum 59: 397-403.
Johansson, Lars, Barbro Andersson, and Tage Eriksson, 1982, Improvement of Anther Culture Technique: Activated Charcoal Bound in Agar Medium in Combination with Liquid Medium and Elevated CO.sub.2 Concentration, Physiologia Plantarum 54: 24-30.
Lu, Chen-Yi, and Trevor A. Thorpe, 1987, Somatic Embryogenesis and Plantlet Regeneration in Cultured Immature Embryos of Picea glauca, Journal of Plant Physiology 128: 297-302.
Murashige, Toshio and Folke Skoog, 1962, A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiologia Plantarum 15: 473-493.
Nagmani, R. and J. M. Bonga, 1985, Embryogenesis in Subcultured Callus of Larix decidua, Canadian Journal of Forest Research 15: 1088-1901.
Nagmani, R. and M. R. Becwar, 1988, Factors Affecting Somatic Embryo Development in Loblolly Pine, Abstracts, 4th International Conifer Tissue Culture Work Group, Aug. 8-12, 1988, Saskatoon Saskatchewan, Canada.
Raghavan, V. N., 1987, Experimental Embryogenesis, p. 100, McMillan, New York.
Schuller, Astrid and Gerhard Reuther, 1989, Response of Abies alba Embryonal-Suspensor Mass to Various Carbohydrate Treatments, Somatic Cell Genetics Working Party S2-04-07 and NATO Advanced Research Workshop on Woody Plant Biology, Institute of Forest Genetics, Placerville, Calif., Oct. 15-19, 1989, (Abstract).
Singh, Hardev, 1978, "Embryo" in Embryology of Gymnosperms, Chapter 11, Gebruder Borntrager, Berlin.
Teasdale, Robert D., Pamela A. Dawson, and H. W. Woolhouse, 1986, Mineral Nutrient Requirements of a Loblolly Pine, (Pinus taeda Cell Suspension Culture, Plant Physiology 82: 942-945.
Von Arnold, Sara, 1987, Improved Efficiency of Somatic Embryogenesis in Mature Embryos of Picea abies (L.) Karst., Journal of Plant Physiology 128: 233-244.
Von Arnold, Sara and Inger Hakman, 1988, Regulation of Somatic Embryo Development in Picea abies by Abscisic Acid (ABA), Journal of Plant Physiology 132: 164-169.
Yeung, Edward C. and D. C. W. Brown, 1982, The Osmotic Enviornment of Developing Embryos of Phaseolus vulgaris, Z. Pfanzenphysiol. Bd., 106 S.: 149-156.
Ziv, Meira and Geula Gadasi, 1986, Enhanced Embryogenesis and Plant Regeneration from Cucumber (Cucumis sativus L.) Callus by Activated Charcoal in Solid/Liquid Double-Layer Cultures, Plant Science 47: 115-122.

Referenced By

Document NumberAssigneeInventorsIssue/Pub Date
AU739097 WEYERHAEUSER CO CARPENTER CAROLYN V et al. Oct 2001
AU764174 WEYERHAEUSER CO CARPENTER CAROLYN V et al. Aug 2003
AU674888 WEYERHAEUSER CO BRYAN CURTIS A et al. Jan 1997
EP1587915 Cellfor Inc. Plamen DENCHEV et al. Jul 2009
EP1498026 WEYERHAEUSER CO CARLSON WILLIAM C et al. Jan 2005
EP1498025 --
US8466086 --
US8744775 --
US5482857 Weyerhaeuser Company Pramod K. Gupta et al. Jan 1996
US5523230 New Zealand Forest Research Institute Limited Dale R. Smith Jun 1996
US5731204 Westvaco Corporation Mark R. Rutter et al. Mar 1998
US5604125 FB Investments Pty Ltd. Robert D. Teasdale Feb 1997
US5491090 Westvaco Corporation Handley, III; Levis W. et al. Feb 1996
US5236841 GUPTA PRAMOD K Pramod K. Gupta et al. Aug 1993
US5464769 University of Saskatchewan Stephen M. Attree et al. Nov 1995
US5413930 Westvaco Corporation Michael R. Becwar et al. May 1995
US5731203 Westvaco Corporation Handley, III; Levis W. Mar 1998
US5985667 University of Saskatchewan Stephen M. Attree et al. Nov 1999
US5731191 Westvaco Corporation Mark R. Rutter et al. Mar 1998
US5310672 Union Camp Corporation Steven R. Wann et al. May 1994
US5294549 Weyerhaeuser Company Gerald S. Pullman et al. Mar 1994
US5608147 Bryan J. Kaphammer Mar 1997
US5856191 Westvaco Corporation Handley, III; Levis W. Jan 1999
US6117678 Weyerhaeuser Company Carolyn V. Carpenter et al. Sep 2000
US5187092 Institute of Paper Science and Technology, Inc. Uddin; M. Rafique Feb 1993
US5563061 Weyerhaeuser Company Pramod K. Gupta Oct 1996
US5506136 Westvaco Corporation Michael R. Becwar et al. Apr 1996
US5677185 Westvaco Corporation Handley, III; Levis W. Oct 1997
US5850032 Union Camp Corporation Steven R. Wann Dec 1998
US6150167 Weyerhaeuser Company Carolyn V. Carpenter et al. Nov 2000
US6180405 Carter Holt Harvey Limited Jennifer Aitken-Christie et al. Jan 2001
US6200809 Cellfor Inc. Krystyna Klimaszewska et al. Mar 2001
US6134830 Weyerhaeuser Company Dorothy Elaine Welty Oct 2000
US7520089 Weyerhaeuser NR Company Jeffrey E. Hartle et al. Apr 2009
US7598073 Weyerhaeuser NR Company Pramod K. Gupta et al. Oct 2009
US6340594 CellFor, Inc. Stephen M. Attree et al. Jan 2002
US6492174 Institute of Paper Science & Technology Gerald S. Pullman et al. Dec 2002
US6372496 CellFor, Inc. Stephen M. Attree et al. Apr 2002
US6682931 MeadWestvaco Corporation Michael Ryan Becwar et al. Jan 2004
US7381562 Weyerhaeuser Company Pramod K. Gupta et al. Jun 2008
US7452722 Weyerhaeuser Company Pramod K. Gupta et al. Nov 2008
US7521237 Weyerhaeuser NR Company Pramod K. Gupta et al. Apr 2009
US7411059 Arborgen, LLC Vincent L. Chiang et al. Aug 2008
US6417001 Carter Holt Harvey Limited Jennifer Aitken-Christie et al. Jul 2002
US6692963 The United States of America as represented by the Secretary of Agriculture Michael Bausher et al. Feb 2004
US6627441 Stephen M. Attree Sep 2003
US7625754 Weyerhaeuser NR Company Pramod K. Gupta et al. Dec 2009
US7888099 Weyerhaeuser NR Company Pramod K. Gupta et al. Feb 2011
US7906334 Cellfor Inc. Plamen Denchev et al. Mar 2011
US7685769 Weyerhaeuser NR Company Amy M Jamruszka-Lewis Mar 2010
US7732205 Weyerhaeuser NR Company Pramod K. Gupta et al. Jun 2010
US7754869 Arborgen, LLC Vincent L. Chiang et al. Jul 2010
US7795029 Cellfor Inc. Shihe Fan et al. Sep 2010
US7950183 Weyerhaeuser NR Company Amy M. Jamruszka-Lewis May 2011
US7951594 Cellfor Inc. Lisheng Kong et al. May 2011
US7960614 Arborgen, LLC Shujun Chang et al. Jun 2011
US8124412 Cellfor Inc. Lisheng Kong et al. Feb 2012
US8181389 Weyerhaeuser NR Company William Carlson et al. May 2012
US8207399 Arborgen Inc. Vincent L. Chiang et al. Jun 2012

Patent Family

The current document is not in a family.