Boliven PRO is more than just patent search

  • Build and save lists using the powerful Lists feature
  • Analyze and download your search results
  • Share patent search results with your clients

Patents »

US5190878: Apparatus for cultivating cells


Filing Information

Inventor(s) Minuth Wilhelm ·
Assignee(s) WILHELM MINUTH ·
Attorney/Agent(s) Lucas & Just ·
Primary Examiner Robert J. Warden ·
Assistant Examiner Trembley; T. A. ·
Application Number US7627729
Filing date 12/14/1990
Issue date 03/02/1993
Predicted expiration date 12/14/2010
U.S. Classifications 435/285  · 435/284  ·
International Classifications C12M 300  ·
Kind CodeA
International Classifications 435170-180;240.1-240.243;284-287;296-301;310-311;313 ·
Foreign Priority DE3923279 - 07/14/1989 ·
15 Claims, No Drawings


The apparatus uses a holding device (2) which has a support ring (3) and a fixed ring (4). A cell substrate (1) is held between support ring (3) and fixed ring (4). The fixed ring (4) has a thickness less than the cylindrical height (3') of the support ring (3) so that only the support ring abuts against the surface of the culture vessel. Since fixing ring (4) does not abut against a surface, there is no pressure on the cell substrate (1). The holding device (2) is held inside the apparatus with space on both the apical and basal sides. Inflow ports (13) and outflow ports (14) are provided for both apical space and basal space to allow for introduction of cell treatment medium.

Independent Claims | See all claims (15)

  1. 1. An apparatus for cultivation of biological cells, comprising:a culture vessel defining an internal space (11, 19, 22, 26);at least one inflow opening (13) communicating with said internal space for introducing a cell treatment medium;at least one outflow opening (14) communicating with said internal space (11, 19, 22, 26) for removing said cell treatment medium;at least one holding device (2) arranged to support a penetrable flat cell substrate (1) with a cell culture thereon and being removably installed in said internal space such that the holding device rests on a surface inside the internal space and the cell treatment medium can come into contact with said cell substrate (1) from both the apical and basal sides;said holding device (2) comprising a supporting ring (3) and a fixing ring (4), said supporting and fixing rings being concentric, and with the cell substrate being fixed between these rings (3, 4);the supporting ring (3) having a supporting ring section (3') encircling said fixing ring (4) and said supporting ring section having an axial height (h);the fixing ring (4) having an axial thickness, the axial thickness of the fixing ring being sufficiently smaller than the axial height (h) of said supporting ring section (3') such that the fixing ring (4), when positioned in said supporting ring, is incapable of abutting the said surface of the internal space of the holding device (2).

Referenced By

Document NumberAssigneeInventorsIssue/Pub Date
EP0773286 Becton Dickinson and Company Oresta N. Fedun Dec 2002
US5776769 Methodist Hospital of Indiana John K. Critser et al. Jul 1998
US6255106 Uwe Marx et al. Jul 2001
US6306644 Uwe Marx et al. Oct 2001
US6329195 ACM-Biotech GmbH Walter Pfaller Dec 2001
US5591636 Precision Instrument Design George M. Grass Jan 1997
US5888807 The Regents of the University of Michigan Bernhard O. Palsson et al. Mar 1999
US5726060 Michael Anthony Bridges Mar 1998
US5635396 Dickinson and Company Becton Oresta N. Fedun Jun 1997
US5599688 Precision Instrument Design George M. Grass Feb 1997
US6197575 Massachusetts Institute of Technology Linda G. Griffith et al. Mar 2001
US6066496 Michael Anthony Bridges May 2000
US5514555 Center for Blood Research, Inc. Timothy A. Springer et al. May 1996
US6054287 Methodist Hospital of Indiana, Inc. Dayong Gao et al. Apr 2000
US6146883 Navicyte, Inc. George M. Grass Nov 2000
US6461861 ABB Limited Henning Schillig et al. Oct 2002
US7541184 Invitrogen Corporation Ronald J. Berenson et al. Jun 2009
US6562616 The General Hospital Corporation Mehmet Toner et al. May 2003
US6905874 XCYTE Therapies, Inc. Ronald Berenson et al. Jun 2005
US6797514 Xcyte Therapies, Inc. Ronald Berenson et al. Sep 2004
US7638325 The Trustees of the University of Pennsylvania Carl June et al. Dec 2009
US6844187 Sefar AG Thomas Wechsler et al. Jan 2005
US6987019 Vitaly Rogalsky Vitaly Rogalsky Jan 2006
US7638326 The Trustees of the University of Pennsylvania Carl June et al. Dec 2009
US6475777 Cell Tissue Progress Farzin Sarem et al. Nov 2002
US6855542 Organogenesis Inc. Paul A. DiMilla et al. Feb 2005
US6867041 XCYTE Therapies, Inc. Ronald Berenson et al. Mar 2005
US6759245 The General Hospital Corporation Mehmet Toner et al. Jul 2004
US7368279 The Regents of The University of Michigan Khalil N. Bitar et al. May 2008
US7572631 Invitrogen Corporation Ronald J Berenson et al. Aug 2009
US7670781 The Trustees of the University of Pennsylvania James Riley et al. Mar 2010
US7713734 Reliance Life Sciences Pvt Ltd Deepa Ghosh et al. May 2010
US7745140 The Trustees of the University of Pennsylvania Carl June et al. Jun 2010
US7754482 The Trustees of the University of Pennsylvania James L. Riley et al. Jul 2010
US7416884 Georgia Tech Research Corporation Christopher Vinson Gemmiti et al. Aug 2008
US7927867 Cascade Life Sciences, Inc. Sophia K Khaldoyanidi Apr 2011
US7977095 Life Technologies Corporation Mark Bonyhadi et al. Jul 2011
US8318479 Massachusetts Institute of Technology Karel Domansky et al. Nov 2012
US8298822 Fraunhofer-Gesellschaft zur Frderung der Angewandten Forschung E.V. Charli Kruse et al. Oct 2012
US8617884 --
US8637307 --

Patent Family

The current document is not in a family.