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US5312910: Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase

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Filing Information

Inventor(s) Ganesh M. Kishore · Dilip M. Shah ·
Assignee(s) Monsanto Company ·
Attorney/Agent(s) Hoerner, Jr.; Dennis R. ·
Primary Examiner David T. Fox ·
Application Number US7941578
Filing date 09/04/1992
Issue date 05/17/1994
Predicted expiration date 05/17/2011
U.S. Classifications 536/232  · 536/234  ·
International Classifications C07H 2104  ·
Kind CodeA
International Classifications 53623.2;23.4;23.6 ·
Related U.S. Application DataThis application is a divisional of and claims priority from co-pending application Ser. No. 550,267 filed Jul. 9. 1990, now U.S. Pat. No. 5,145,783, which is a continuation-in-part of application Ser. No. 179,245, filed Apr. 22, 1988, now U.S. Pat. No. 4,971,908, which is a continuation-in-part of application Ser. No. 054,337, filed May 26, 1987, now abandoned.
6 Claims, No Drawings


Abstract

Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphosate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a conserved sequence found between positions 80 and 120 in the mature wild-type EPSP synthase.

Independent Claims | See all claims (6)

  1. 1. A plant gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase, said EPSP synthase having the amino acid sequence:-L-G-N-A-A-T-A-between positions 80 and 120 in the mature EPSP synthase sequence.
  2. 2. A DNA molecule encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase, said EPSP synthase having the amino acid sequence:-L-G-N-A-A-T-A-between positions 80 and 120 in the mature EPSP synthase sequence.
  3. 5. A method of using a planted crop seed or plant which is glyphosate tolerant to selectively control weeds in a field containing said planted crop seed or plant, said method comprising the steps of:a) planting said crop seed or plant which is glyphosate tolerant as a result of a chimeric gene being inserted into said crop seed or plant, said chimeric gene havingi) a promoter sequence which functions in plant cells,ii) a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell and said glyphosate-tolerant EPSP synthase has the sequence:-L-G-N-A-A-T-A-between positions 80 and 120 of the mature EPSP synthase sequence, andiii) a 3' non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of multiple adenine nucleotides to the 3' end of the RNA, where the promoter is adapted to cause sufficient expression of the fusion polypeptide to enhance the glyphosate resistance of a plant cell transformed with said gene; andb) applying to said crop and weeds in said field a sufficient amount of glyphosate to control said weeds without significantly affecting said crop.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US4769061 Calgene Inc. Comai Sep 1988
US4971908 Monsanto Company Kishore et al. Nov 1990
US5145783 Monsanto Company Kishore et al. Sep 1992

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Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
US5145783 Monsanto Company Ganesh M. Kishore et al. Sep 1992
US5312910 Monsanto Company Ganesh M. Kishore et al. May 1994