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US5427937: Hookworm anticoagulant

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Filing Information

Inventor(s) Michael Cappello · Peter J. Hotez · Frank F. Richards · John M. Hawdon ·
Assignee(s) None listed in document.
Attorney/Agent(s) Krinsky; Mary M.St. Onge Steward Johnston & Reens ·
Primary Examiner Robert A. Wax ·
Assistant Examiner Hyosuk Kim ·
Application Number US8055988
Filing date 04/30/1993
Issue date 06/27/1995
Predicted expiration date 04/30/2013
U.S. Classifications 435/212  · 435/214  ·
International Classifications C12N 948  ·
Kind CodeA
International Classifications 435212;219 ·
5 Claims, No Drawings


Abstract

A soluble anticoagulant protein isolated and purified from Ancylostoma hookworms markedly prolongs both the prothrombin time and partial thromboplastin time in clotting assays. The protein has an apparent molecular weight of about 6500 daltons and exhibits amino acid sequence homology to the Kunitz-type serine protease inhibitor family. Chromogenic peptide substrate and clotting time assays indicate that the protein inhibits extrinsic pathway clotting factor VIIa, the enzyme responsible for initiating the human coagulation cascade, and factor Xa in the common pathway of the coagulation cascade.

Independent Claims | See all claims (5)

  1. 1. An anticoagulant composition comprising a purified soluble protein isolated from hookworms selected from the group consisting of Ancylostoma duodenale, Ancylostoma ceylanicum, Necator americanus, and Ancylostoma caninum, wherein said protein prolongs the prothrombin time and partial thromboplastin time, and wherein said protein inhibits clotting factor Xa, does not inhibit thrombin or clotting factors II and V, and also wherein said protein contains the amino acid sequence Tyr-Pro-Glu-Cys-Gly-Glu-Asn-Cys-Gly-Leu.
  2. 3. A composition according to clam 1 wherein said protein is isolated and purified from Ancylostoma caninum.
  3. 5.5. An anticoagulant composition comprising a purified soluble protein isolated from Ancylostoma caninum or Necator americanus, wherein said protein inhibits clotting factor Xa but does not inhibit thrombin or clotting factors II and V and also wherein said protein contains the amino acid sequence Tyr-Pro-Glu-Cys-Gly-Glu-Asn-Cys-Gly-Leu.

References Cited

The current document has no citations.

Other Publications

Capello et al. "Ancylostoma Factor Xa . . . " J. Inf. Dis. vol. 167 pp. 1474-1477 Jun., 1993.

Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
CA2161796 CAPPELLO MICHAEL et al. Nov 1994
US5427937 Michael Cappello et al. Jun 1995
EP0697857 YALE UNIVERSITY Michael CAPPELLO et al. Feb 1996