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US5561107: Method of enhancing wound healing by stimulating fibroblast and keratinocyte growth in vivo, utilizing amphipathic peptides

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Filing Information

Inventor(s) Jesse M. Jaynes · Gordon R. Julian ·
Assignee(s) Demeter Biotechnologies, Ltd. ·
Attorney/Agent(s) Rothwell, Figg, Ernst & Kurz ·
Primary Examiner Jeffrey E. Russel ·
Application Number US8231730
Filing date 04/20/1994
Issue date 10/01/1996
Predicted expiration date 06/04/2013
U.S. Classifications 514/12  · 514/13  ·
International Classifications A61K 3810  ·
Kind CodeA
International Classifications 530324;325;326;327;345 ·
Related U.S. Application DataCROSS-REFERENCE TO RELATED APPLICATIONS
This is a continuation-in-part of U.S. patent application Ser. No. 08/225,476 filed Apr. 8, 1994 in the names of Jesse M. Jaynes and Gordon R. Julian for "METHOD OF COMBATTING MAMMALIAN NEOPLASIA, AND LYTIC PEPTIDES THEREFOR", now abandoned, which in turn is a continuation-in-part of 08/039,620 filed Jun. 4, 1993 in the names of Jesse M. Jaynes and Gordon R. Julian, now abandoned, and is also a continuation-in-part of U.S. patent application Ser. No. 08/148,889 filed Nov. 8, 1993 in the name of Gordon R. Julian, now abandoned, as well as a continuation-in-part of U.S. patent application Ser. No. 08/148,491 filed Nov. 8, 1993 in the name of Gordon R. Julian, now abandoned.
13 Claims, No Drawings


Abstract

A method of treating a wound of a mammalian subject in need of such treatment, to promote healing thereof, comprising administering to the subject, e.g., to the wound locus, a composition comprising a fibroblast and keratinocyte proliferatingly effective amount of an amphipathic peptide, preferably an amphipathic peptide which is antimicrobially effective at such locus. A method is also disclosed of stimulating the accelerated growth of dermal tissue in a tissue culture containing same, comprising applying to the tissue culture a fibroblast and keratinocyte proliferatingly effective amount of an amphipathic peptide, by which the dermal tissue may be grown to produce skin for skin grafting purposes, utilizing a dermal tissue culture containing dermal tissue material of a skin graft recipient of such skin. Novel amphipathic peptides suitable for use in such methods are disclosed.

Independent Claims | See all claims (13)

  1. 1. A method of stimulating the accelerated growth of dermal tissue in a tissue culture containing the same, comprising applying to the tissue culture a fibroblast and keratinocyte proliferatingly effective amount of an amphipathic peptide having a sequence selected from the group consistinq of SEQ ID NOs. 41 to 46.
  2. 2. A method of promoting wound healing in a mammalian subject having a wound in need of treatment comprising administering to said subject a composition of a fibroblast and keratinocyte proliferatingly effective amount of an amphipathic peptide, wherein the amphipathic peptide comprises a peptide having a sequence selected from SEQ ID NOs. 41 to 46.
  3. 3. A method of promoting wound healing in a mammalian subject having a wound in need of treatment comprising administering to said subject a composition of a fibroblast and keratinocyte proliferatingly effective amount of an amphipathic peptide of a sequence selected from SEQ ID NOs. 41 to 46, wherein the amphipathic peptides has been modified to provide enhanced proteolytic resistance to the peptide.
  4. 11. An amphipathic peptide comprising a peptide having a sequence selected from SEQ ID NOs. 41 to 46.

References Cited

U.S. Patent Documents

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Foreign Patent Documents

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Other Publications

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Jaynes, J. M., et al. "In Vitro Cytocidal Effect Of Lytic Peptides On Several Transformed Mammalian Cell Lines", Peptide Research, 2:157-160 (1989).
Jaynes, J. M. "Lytic Peptides Portend An Innovation Age In The Management And Treatment Of Human Disease", Drug News and Perspectives, 3:69-78 (1990).
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"Preliminary Experimental Anti-Cancer Activity Of Cecropin B," Proceedings of the American Association for Cancer Research 35:410 (1994).

Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
US5561107 Demeter Biotechnologies, Ltd. Jesse M. Jaynes et al. Oct 1996
US5773413 Demeter Biotechnologies, Ltd. Jesse M. Jaynes et al. Jun 1998