Join
today

Boliven PRO is more than just patent search

  • Build and save lists using the powerful Lists feature
  • Analyze and download your search results
  • Share patent search results with your clients

Patents »

US5608147: tfdA gene selectable markers in plants and the use thereof

Share

Filing Information

Inventor(s) Bryan J. Kaphammer ·
Assignee(s) None listed in document.
Attorney/Agent(s) Dechert Price & Rhoads ·
Primary Examiner Douglas W. Robinson ·
Assistant Examiner Elizabeth F. McElwain ·
Application Number US8358117
Filing date 12/15/1994
Issue date 03/04/1997
Predicted expiration date 01/11/2014
U.S. Classifications 800/205  · 800/250  ·
International Classifications A01H 500  ·
Kind CodeA
International Classifications 800205;250;255 ·
Related U.S. Application DataCROSS-REFERENCE TO RELATED APPLICATIONS
This is a continuation-in-part of U.S. application Ser. No. 08/179,667, filed Jan. 11, 1994, now abandoned, the contents of which are fully incorporated by reference herein.
33 Claims, No Drawings


Abstract

The present invention relates, in general, to transgenic plant cells and plants. In particular, the present invention relates to 1) a method of selecting for a transgenic plant cell comprising transforming one or more plant cells with a tfdA gene; 2) a plant cell comprising a tfdA gene wherein said plant cell is free of other foreign marker genes; and 3) a sweetgum plant cell comprising a tfdA gene.

Independent Claims | See all claims (33)

  1. 1. A method of selecting for a transformed plant cell comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene expressible in said plant cell, andb) directly selecting on a culture medium the resulting transformed plant cell with an amount of 2,4-dichlorophenoxyacetic acid as a selectable agent, the 2,4-dichlorophenoxyacetic acid being in an amount which inhibits adventitious shoot regeneration of non-transformed plant cells, wherein adventitious shoot regeneration from said transformed plant cell is not inhibited.
  2. 6. A method of selecting for a transformed plant comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene expressible in said plant cell,b) directly selecting on a culture medium the resulting transformed plant cell with an amount of 2,4-dichlorophenoxyacetic acid as a selectable agent, the 2,4-dichlorophenoxyacetic acid being in an amount which inhibits adventitious shoot regeneration of non-transformed plant cells, wherein adventitious shoot regeneration from said transformed plant cell is not inhibited, andc) regenerating the selected plant cell into a plant.
  3. 11. A plant cell comprising a tfdA gene expressible in said plant cell wherein said plant cell is free of other foreign selectable marker genes.
  4. 19. A sweetgum plant cell comprising a tfdA gene expressible in said plant cell.
  5. 24. A method of producing a hardwood plantation comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene expressible in said plant cell;b) regenerating said plant cell into a plant;c) culturing said regenerated plant with an amount of 2,4-dichlorophenoxyacetic acid which will kill non-transformed plants;d) selecting a plant exhibiting growth;e) propagating said plant to produce many plants;f) inducing root formation in said plants;g) growing said rooted plants to planting stock size;h) planting the planting stock size plants in a sheared, defoliated site, andi) applying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control.
  6. 25. A method of producing a hardwood plantation comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene and at least one second gene encoding a foreign selectable marker expressible in said plant cell;b) culturing said plant cell with an amount of a chemical which will inhibit adventitious shoot regeneration of plant cells not transformed with the foreign selectable marker gene;c) selecting a plant cell exhibiting adventitious shoot regeneration;d) regenerating said plant cell into a plant;e) propagating said plant to produce many plants;f) inducing root formation in said plants;g) growing said rooted plants to planting stock size;h) planting the planting stock size plants in a sheared, defoliated site, andi) applying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control.
  7. 26. A method for producing a hardwood plantation comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene and at least one second gene encoding a foreign selectable marker expressible in said plant cell;b) culturing said plant cell with an amount of a chemical which will inhibit adventitious shoot regeneration of plant cells not transformed with the foreign selectable marker gene;c) selecting a plant cell exhibiting adventitious shoot regeneration;d) regenerating said plant cell into a plant;e) culturing said regenerated plant with an amount of 2,4-dichlorophenoxyacetic acid which will kill non-transformed plants;f) selecting a plant exhibiting growth;g) propagating said plant to produce many plants;h) inducing root formation in said plants;i) growing said rooted plants to planting stock size;j) planting the planting stock size plants in a sheared, defoliated site, andk) applying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control.
  8. 30. A method of producing a hardwood plantation comprising:planting stock size plants in a sheared, defoliated site, andapplying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control,wherein said plant is a hardwood comprising a tfdA gene.
  9. 32. A plantation of hardwood trees comprising hardwood trees comprising a tfdA gene.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US4459355 International Paper Company Cello et al. Jul 1984
US4535060 Calgene, Inc. Comai Aug 1985
US4658082 Atlantic Richfield Company Simpson et al. Apr 1987
US4761373 Molecular Genetics, Inc. Anderson et al. Aug 1988
US4769061 Calgene Inc. Comai Sep 1988
US4795855 FILLATTI JOANNE Fillatti et al. Jan 1989
US4940835 Monsanto Company Shah et al. Jul 1990
US4975374 The General Hospital Corporation Goodman et al. Dec 1990
US5036007 Weyerhaeuser Company Gupta et al. Jul 1991
US5094945 Calgene, Inc. Comai Mar 1992
US5145783 Monsanto Company Kishore et al. Sep 1992
US5149645 Rijksuniversiteit Leiden Hoekema et al. Sep 1992
US5225341 The Regents of the University of California Yoder et al. Jul 1993

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
EP0154204MGI PHARMA, INC.Sep 1985
EP0492113AMERICAN CYANAMID COMPANYJul 1992
EP0530129SANDOZ LTD.Mar 1993

Other Publications

Davis, D. G. and Olson, P.A., "Organogenesis in Leafy Spurge (Euphorbia esula L.)," In Vitro Cell Dev. Biol. 29P:97-101 (Jul. 1993).
Sullivan, J. and Lagrimini, L. M., "Transformation of Liquidambar styraciflua using Agrobacterium tumefaciens," Plant Cell Reports 12:303-306 (Apr. 1993).
De Block, M., "Factors Influencing the Tissue Culture and the Agrobacterium tumefaciens-Mediated Transformation of Hybrid Aspen and Poplar Clones," Plant Physiol. 93:1110-1116 (1990).
Fillatti, J. J., et al., "Agrobacterium Mediated Transformation and Regeneration of Populus," Mol. Gen. Genet. 206:192-199 (1987).
Hajela, R. K., et al., "A Simple Transformation System Using Adventitious Shoot Multiplication of Juneberry," HortScience 28(4):330-332 (1993).
Barton et al., Bacillus thuringiensis delta-Endotoxin Expressed in Transgenic Nicotiana tabacum provides Resistance to Lepidopteran Insects, Plant Physiol. 85:1103-1109 (1987).
Bayley et al., "Engineering 2,4-D resistance into cotton", Theor. Appl. Genet. 83:645-649 (1992).
Bozhkov et al., "A pronounced synergistic effect of abscisic acid and 6-benzyladenine on Norway spruce (Picea abies L. Karst) somatic embryo maturation", Plant Cell Rep. 11:386-389 (1992).
Brand & Lineberger, "In vitro adventitious shoot formation on mature-phase leaves and petioles of Liquidambar Styraciflua L. ", Plant Sci. 57:173-179 (1988).
Brasileiro et al., "Expression of the mutant Arabidopsis thaliana acetolactate synthase gene confers chlorosulfuron resistance to transgenic poplar plants", Transgenic Res. 1:133-141 (1992).
Bueno et al., "Plant regeneration through somatic embryogenesis in Quercus suber", Phys. Plant. 85:30-34 (1992).
Chalupa, V., "Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L.) and linden (Tilia cordata Mill.)", Plant Cell Rep. 9:398-401 (1990).
Chen & Stomp, "Transformation of Liquidamber styraciflua L. (Sweetgum) using Agrobacterium Tumefaciens," Proceedings 21st Southern Forest Tree Improvement Conference, (Jun. 17-20, 1991), Abstract, p. 315.
Crossway et al., "Integration of foreign DNA following microinjection of tobacco mesophyl protoplasts", Mol. Gen. Genet. 202:179-185 (1986).
De Block, M., Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens, Theor. Appl. Genet. 76:767-774 (1988).
Fasolo et al., "Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars", Plant Cell, Tissue and Org. Cult. l6:75-87 (1989).
Gingas & Lineberger, "Asexual embryogenesis and plant regeneration in Quercus" Plant Cell, Tissue and Org. Cult. 17:191-203 (1989).
Gingas, V. M., "Asexual Embryogenesis and Plant Regeneration from Male Catkins of Quercus", Hort. Sci. 26:1217-1218 (1991).
Harker et al., "Phenoxyacetic Acid Degradation by the 2,4-Dichlorophenoxyacetic Acid (TFD) Pathway of Plasmid pJP4: Mapping and Characterization of the TFD Regulatory Gene, tfdR", J. Bacteriol. 171:314-320 (Jan. 1989).
Hinchee et al., "Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA Transfer", Bio/Technology 6:915-922 (Aug. 1988).
Hughes, K., Selection of Herbicide Resistance, Handbook of Plant Cell Culture, vol. 1 ( ed. Evans et al.) Mac Millan Publ., NY, pp. 442-460 (1983).
Hunt, Hardwood Short Course, "Commercial Hardwood Planting", North Carolina State University, pp. 63-67 (May 15-17, 1973).
James et al., "Factors Affecting High Frequency Plant Regeneration from Apple Leaf Tissues Cultured in vitro", J. Plant Physiol. 132:148-154 (1988).
Jordan et al., "Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer", Plant Cell Rep. 7:281-284 (1988).
Kvaalen & von Arnold, "Effects of various partial pressures of oxygen and carbon dioxide on different stages of somatic embryogenesis in Picea abies", Plant Cell, Tissue and Org. Cult. 27:49-57 (1991).
Leple et al., "Transgenic poplars: expression of chimeric genes using four different constructs", Plant Cell Rep. 11:137-141 (1992).
Lyon et al., "Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid", Plant Mol. Biol. 13:533-540 (1989).
Malac & Heeren, "Hardwood Plantation Management", Southern J. Appl. Forest. 3:3-6 (Feb. 1979).
McGranahan et al., "Agrobacterium-mediated transformation of walnut somatic embryos and regeneration of transgenic plants", Bio/Technology 6:800-804 (Jul. 1988).
Michler & Bauer, "High frequency somatic embryogenesis from leaf tissues of Populus spp.", Plant Sci. 77:111-118 (1991).
Noh & Minocha, "High efficiency shoot regeneration from callus of quaking aspen (Populus tremuloides Michx.)", Plant Cell Rep. 5:464-467 (1986).
Park & Son, "In vitro organogenesis and somatic embryogenesis from punctured leaf of Populus nigra X P. maximowiczii", Plant Cell, Tissue and Organ Culture 15:95-105 (1988).
Predieri & Malavasi, "High-frequency shoot regeneration from leaves of the apple rootstock M26 (Malus pumila Mill.)", Plant Cell, Tissue and Organ Culture 17:133-142 (1989).
Roberts et al., "Synchronous and high frequency germination of interior spruce somatic embryos following partial drying at high relative humidity", Can. J. Bot. 68:1086-1090 (1990).
Rowland & Ogden, "Use of a cytokinin conjugate for efficient shoot regeneration from leaf sections of highbush blueberry", Hort. Sci. 27:1127-1129 (Oct. 1992).
Stalker et al., "Purification and Properties of a Nitrilase Specific for the Herbicide Bromoxynil and Corresponding Nucleotide Sequence Analysis of the bxn Gene", J. Biol. Chem. 263:6310-6314 (May 5, 1988).
Stomp et al., "Extended Host Range of Agrobacterium tumefaciens in the Genus Pinus", Plant Physiology 92:1226-1232 (1990).
Streber & Willmitzer, "Transgenic tobacco plants expressing a bacterial detoxifying enzyme are resistant to 2,4-D", Bio/Technology 7:811-816 (Aug. 1989).
Streber et al., "Analysis, Cloning, and High-Level Expression of 2,4-Dichlorophenoxyacetate Monooxygenase Gene tfdA of Alcaligenes eutrophus JMP134", J. of Bacteriology 169:2950-2955 (Jul. 1987).
Thillet et al., "Site-directed Mutagenesis of Mouse Dihydrofolate Reductase", J. Biol. Chem. 263:12500-12508 (Sep. 5, 1988).
Tremblay & Tremblay, "Carbohydrate requirements for the development of black spruce (Picea mariana (Mill.) B.S.P.) and red spruce (P. rubens Sarg.) somatic embryos", Plant Cell, Tissue, and Org. Cult. 27:95-103 (1991).
Valobra & James, "In vitro shoot regeneration from leaf discs of Betula pendula `Dalecarlica` EM 85", Plant Cell, Tissue and Org. Cult. 21:51-54 (1990).
Wetzstein et al., "Further Characterization of somatic embryogenesis and plantlet regeneration in pecan (Carya Illinoensis)", Plant Sci. 64:193-201 (1989).

Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
ZA9410322 UNION CAMP CORP KAPHAMMER BRYAN J Sep 1995
US5608147 Bryan J. Kaphammer Mar 1997