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US5608147: tfdA gene selectable markers in plants and the use thereof

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Filing Information

Inventor(s) Bryan J. Kaphammer ·
Assignee(s) None listed in document.
Attorney/Agent(s) Dechert Price & Rhoads ·
Primary Examiner Douglas W. Robinson ·
Assistant Examiner Elizabeth F. McElwain ·
Application Number US8358117
Filing date 12/15/1994
Issue date 03/04/1997
Predicted expiration date 01/11/2014
U.S. Classifications 800/205  · 800/250  ·
International Classifications A01H 500  ·
Kind CodeA
International Classifications 800205;250;255 ·
Related U.S. Application DataCROSS-REFERENCE TO RELATED APPLICATIONS
This is a continuation-in-part of U.S. application Ser. No. 08/179,667, filed Jan. 11, 1994, now abandoned, the contents of which are fully incorporated by reference herein.
33 Claims, No Drawings


Abstract

The present invention relates, in general, to transgenic plant cells and plants. In particular, the present invention relates to 1) a method of selecting for a transgenic plant cell comprising transforming one or more plant cells with a tfdA gene; 2) a plant cell comprising a tfdA gene wherein said plant cell is free of other foreign marker genes; and 3) a sweetgum plant cell comprising a tfdA gene.

Independent Claims | See all claims (33)

  1. 1. A method of selecting for a transformed plant cell comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene expressible in said plant cell, andb) directly selecting on a culture medium the resulting transformed plant cell with an amount of 2,4-dichlorophenoxyacetic acid as a selectable agent, the 2,4-dichlorophenoxyacetic acid being in an amount which inhibits adventitious shoot regeneration of non-transformed plant cells, wherein adventitious shoot regeneration from said transformed plant cell is not inhibited.
  2. 6. A method of selecting for a transformed plant comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene expressible in said plant cell,b) directly selecting on a culture medium the resulting transformed plant cell with an amount of 2,4-dichlorophenoxyacetic acid as a selectable agent, the 2,4-dichlorophenoxyacetic acid being in an amount which inhibits adventitious shoot regeneration of non-transformed plant cells, wherein adventitious shoot regeneration from said transformed plant cell is not inhibited, andc) regenerating the selected plant cell into a plant.
  3. 11. A plant cell comprising a tfdA gene expressible in said plant cell wherein said plant cell is free of other foreign selectable marker genes.
  4. 19. A sweetgum plant cell comprising a tfdA gene expressible in said plant cell.
  5. 24. A method of producing a hardwood plantation comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene expressible in said plant cell;b) regenerating said plant cell into a plant;c) culturing said regenerated plant with an amount of 2,4-dichlorophenoxyacetic acid which will kill non-transformed plants;d) selecting a plant exhibiting growth;e) propagating said plant to produce many plants;f) inducing root formation in said plants;g) growing said rooted plants to planting stock size;h) planting the planting stock size plants in a sheared, defoliated site, andi) applying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control.
  6. 25. A method of producing a hardwood plantation comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene and at least one second gene encoding a foreign selectable marker expressible in said plant cell;b) culturing said plant cell with an amount of a chemical which will inhibit adventitious shoot regeneration of plant cells not transformed with the foreign selectable marker gene;c) selecting a plant cell exhibiting adventitious shoot regeneration;d) regenerating said plant cell into a plant;e) propagating said plant to produce many plants;f) inducing root formation in said plants;g) growing said rooted plants to planting stock size;h) planting the planting stock size plants in a sheared, defoliated site, andi) applying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control.
  7. 26. A method for producing a hardwood plantation comprising:a) transforming one or more plant cells with a polynucleotide comprising a tfdA gene and at least one second gene encoding a foreign selectable marker expressible in said plant cell;b) culturing said plant cell with an amount of a chemical which will inhibit adventitious shoot regeneration of plant cells not transformed with the foreign selectable marker gene;c) selecting a plant cell exhibiting adventitious shoot regeneration;d) regenerating said plant cell into a plant;e) culturing said regenerated plant with an amount of 2,4-dichlorophenoxyacetic acid which will kill non-transformed plants;f) selecting a plant exhibiting growth;g) propagating said plant to produce many plants;h) inducing root formation in said plants;i) growing said rooted plants to planting stock size;j) planting the planting stock size plants in a sheared, defoliated site, andk) applying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control.
  8. 30. A method of producing a hardwood plantation comprising:planting stock size plants in a sheared, defoliated site, andapplying 2,4-dichlorophenoxyacetic acid over the entire site to suppress competition growth until the planting stock can thrive without competition growth control,wherein said plant is a hardwood comprising a tfdA gene.
  9. 32. A plantation of hardwood trees comprising hardwood trees comprising a tfdA gene.

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Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
ZA9410322 UNION CAMP CORP KAPHAMMER BRYAN J Sep 1995
US5608147 Bryan J. Kaphammer Mar 1997