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US5670488: Adenovirus vector for gene therapy

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Filing Information

Inventor(s) Richard J. Gregory · Donna Armentano · Larry A. Couture · Alan E. Smith ·
Assignee(s) Genzyme Corporation ·
Attorney/Agent(s) Brumbaugh, Graves Donohue & Raymond ·
Primary Examiner Deborah Crouch ·
Application Number US8136742
Filing date 10/13/1993
Issue date 09/23/1997
Predicted expiration date 12/03/2012
U.S. Classifications 514/44  · 424/932  ·
International Classifications A61K 4800  ·
Kind CodeA
International Classifications 435320.1 ·
Related U.S. Application DataRELATED APPLICATIONS
This application is a continuation-in-part of application U.S. Ser. No. 07/985,478, filed Dec. 3, 1992, abandoned.
19 Claims, No Drawings


Abstract

Gene Therapy vectors, which are especially useful for cystic fibrosis, and methods for using the vectors are disclosed.

Independent Claims | See all claims (19)

  1. 1. An adenoviral vector comprising an adenovirus genome from which one or more of the E4 open reading frames has been deleted, but retaining sufficient E4 sequences to promote virus replication in vitro, and additionally comprising a DNA sequence of interest operably linked to expression control sequences and inserted into said adenoviral genome.
  2. 13. A method for providing cystic fibrosis transmembrane conductance regulator protein to airway epithelial cells of a cystic fibrosis patient comprising administering directly to airway epithelial cells of the patient an adenoviral vector, said vector comprising an adenovirus genome from which one or more E4 open reading frames has been deleted, but retaining sufficient E4 sequences to promote virus replication in vitro, and additionally comprising a DNA sequence encoding cystic fibrosis transmembrane regulator protein operably linked to expression control sequences and inserted into the E1 region said adenoviral genome, under conditions whereby the DNA sequence encoding cystic fibrosis transmembrane regulator protein is expressed and a functional chloride ion channel is produced in the airway epithelial cells of the patient.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US4920209 American Home Products Corporation Davis Apr 1990

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
EP0185573INSTITUT PASTEURJun 1986
EP0446017GENZYME CORPORATIONSep 1990
WO20WO9102796Aug 1990
WO20WO9312240Dec 1992
WO20WO9312756Dec 1992
WO20WO9110734Dec 1992
WO199319191Sep 1993

Other Publications

Zabner, J. et al. (1993) "Adenovirus-Mediated Gene Transfer Transiently Corrects the Chloride Transport Defect in Nasal Epithelia of Patients with Cystic Fibrosis" Cell 75:207-216.
Rosenfeld, M.A. et al. (1992) "In Vivo Transfer of the Human Cystic Fibrosis Transmembrane Conductance Regulator Gene to the Airway Epithelium" Cell 68: 143-155.
Sassone-Corsi, P. et al. (1983) "Far upstream sequences are required for efficient transcription from the adenovirus-2 E1A transcription unit" Nucleic Acid Research 11:8735-8745.
Yoshimura, K. et al. (1993) "Adenovirus-mediated Augmentation of Cell Transfection with Unmodified Plasmid Vectors" J. Biol. Chem. 268(4):2300-2303.
Engelhardt, J.F. et al. (1993) "Direct Gene Transfer of Human CFTR into Human Bronchial Epithelia of Xenografts with E1-deleted Adenovirus" Nat. Gen. 4:27-34.
Flotte, T.R. et al. (1993) "Expression of the Cystic Fibrosis Transmembrane Conductance Regulator from a Novel Adeno-associated Virus Promoter" J. Biol. Chem. 268(5):3781-3790.
Johnson, L.G. et al. (1992) "Efficiency of Gene Transfer for Restoration of Normal Airway Epithelial Function in Cystic Fibrosis" Nat. Gen. 2:21-25.
Welsh, M.J. and Smith, A.E. (1993) "Molecular Mechanisms of CFTR Chloride Channel Dysfunction in Cystic Fibrosis" Cell 73:1251-1254.
Hyde, S.C. et al. (1993) "Correction of the Ion Transport Defect in Cystic Fibrosis Transgenic Mice by Gene Therapy" Nature 362:250-255.
Smith, A.E. (1992) "Emerging Therapies for Cystic Fibrosis" Section V-Topics in Biology in Ann. Rep. Med. Chem. 27:235-243.
Yoshimura, K. et al. (1992) "Expression of the Human Cystic Fibrosis Transmembrane Conductance Regulator Gene in the Mouse Lung after in Vivo Intratracheal Plasmid-Mediated Gene Transfer" Nucleic Acids Res. 20:(12):3233-3240.
Rosenfeld, M.A. et al. (1991) "Adenovirus-Mediated Transfer of a Recombinant .alpha.-1-Antitrypsin Gene to the Lung Epithelium in Vivo" Science 252:431-434.
Rosenfeld, M.A. et al. (1991) "In Vivo Transfer of the Human Cystic Fibrosis Gene to the Respiratory Epithelium" Clin. Res. 39(2):311A.
Anderson, M.P. et al. (1991) "Generation of cAMP-Activated Chloride Currents by Expression of CFTR" Science 251:679-682.
Kartner, N. et al. (1991) "Expression of the Cystic Fibrosis Gene in Non-Epithelial Invertebrate Cells Produces a Regulated Anion Conductance" Cell 64:681-691.
Anderson, M.P. et al. (1991) "Demonstration That CFTR Is a Chloride Channel by Alteration of Its Anion Selectivity" Science 253:202-205.
Tabcharani, J.A. et al. (1991) "Phosphorylation-Regulated C1-Channel in CHO Cells Stabley Expressing the Cystic Fibrosis Gene" Nature 352:628-631.
Rich, D.P. et al. (1991) "Effect of Deleting the R Domain on CFTR-Generated Chloride Channels" Science 253:205-207.
Berger, H.A. et al. (1991) "Identification and Regulation of the Cystic Fibrosis Transmembrane Conductance Regulator-Generated Chloride Channel" J. Clin. Invest. 88:1422-1431.
Drumm, M.L. et al. (1990) "Correction of the Cystic Fibrosis Defect In Vitro By Retrovirus-Mediated Gene Transfer" Cell 1227-1233.
Gregory, R.J. et al. (1990) "Expression and Characterization of the Cystic Fibrosis Transmembrane Conductance Regulator" Nature 347:382-386.
Rich, D.P. et al. (1990) "Expression of the Cystic Fibrosis Transmembrane Conductance Regulator Corrects Defective Chloride Channel Regulation in Cystic Fibrosis Airway Epithelial Cells" Nature 347:358-363.
Huang, M.T.F. et al. (1990) "Intervening Sequences Increase Efficiency of RNA 3'Processing and Accumulation of Cytoplasmic RNA" Nucleic Acids Res. 18(4):937-947.
Riordan, J.R. et al. (1989) "Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNA" Science 245:1066-1073.
Rommens, J.H. et al. (1989) "Identification of the Cystic Fibrosis Gene: Chromosome Walking and Jumping" Science 245: 1059-1065.
Kerem, B.S. et al. (1989) "Identification of the Cystic Fibrosis Gene: Genetic Analysis" Science 245:1073-1080.
Berker (1988) Biotechniques 6, 616-627.

Referenced By

Document NumberAssigneeInventorsIssue/Pub Date
AU772544 ONYX PHARMA INC HERMISTON TERRY et al. Apr 2004
EP1702983 MEDICAL UNIVERSITY OF SOUTH CAROLINA James S. Norris et al. Sep 2006
EP1027069 GENZYME CORPORATION Nelson S. YEW et al. Jul 2006
EP1135514 ONYX PHARMACEUTICALS, INC. Terry HERMISTON et al. May 2009
EP1117781 Incyte Pharmaceuticals, Inc. Janice AU-YOUNG et al. Jul 2001
US8278098 Vegenics Pty Limited Kari Alitalo et al. Oct 2012
US8828378 --
US8835614 --
US6328958 Duke University Andrea Amalfitano et al. Dec 2001
US6040174 Transgene S.A. Jean-Luc Imler et al. Mar 2000
US6271359 MUSC Foundation for Research Development James Norris et al. Aug 2001
US6238893 Introgene B.V. Robert Cornelis Hoeben et al. May 2001
US6033908 b.v. IntroGene Abraham Bout et al. Mar 2000
US6066626 Genzyme Corporation Nelson S. Yew et al. May 2000
US6245330 Aventis Pharma S.A. Philippe Horellou et al. Jun 2001
US5994128 IntroGene B.V. Frits Jacobus Fallaux et al. Nov 1999
US5985846 Baylor College of Medicine Stefan Kochanek et al. Nov 1999
US6265212 Introgene B.V. Frits J. Fallaux et al. Jul 2001
US6312946 Rhone-Poulenc Rorer S.A. Patrice Yeh et al. Nov 2001
US6204052 Introgene B.V. Abraham Bout et al. Mar 2001
US6133028 Transgene S.A. Jean-Luc Imler et al. Oct 2000
US5998384 Hisamitsu Pharmaceutical Co., Inc. Takashi Shimada et al. Dec 1999
US5877011 Genzyme Corporation Donna Armentano et al. Mar 1999
US6207648 Trustees of Boston University David J. Waxman et al. Mar 2001
US6306652 IntroGene B.V. Frits Jacobus Fallaux et al. Oct 2001
US5962429 University of Iowa Michael J. Welsh et al. Oct 1999
US5935935 Genetic Therapy, Inc. Sheila Connelly et al. Aug 1999
US6080569 Merck & Co., Inc. Frank L. Graham et al. Jun 2000
US6265218 Roche Diagnostics GmbH Stefan Seeber et al. Jul 2001
US5981225 Baylor College of Medicine Stefan Kochanek et al. Nov 1999
US6303362 The Board of Trustees of the Leland Stanford Junior University Mark A. Kay et al. Oct 2001
US6395519 IntroGene B.V. Frits J. Fallaux et al. May 2002
US6464998 Aventis Pharma S.A. Yves Beuzard et al. Oct 2002
US6379943 Merck & Co., Inc. Frank L. Graham et al. Apr 2002
US6770632 The General Hospital Corporation Manish Aghi et al. Aug 2004
US6803194 HK Pharmaceuticals, Inc. James G. Keck et al. Oct 2004
US7067309 Transgene S.A. Jean-Luc Imler et al. Jun 2006
US7318919 Genzyme Corporation Richard Gregory et al. Jan 2008
US6544781 The General Hospital Corporation Xiaoqun Jiang et al. Apr 2003
US7045344 The Board of Trustees of the LeLand Stanford Junior University Mark A. Kay et al. May 2006
US7357795 Olympus Corporation Hinako Kaji et al. Apr 2008
US7510875 Introgen Therapuetics, Inc. Shuyuan Zhang et al. Mar 2009
US6670188 Crucell Holland B.V. Ronald Vogels et al. Dec 2003
US6838285 Becton Dickinson Andrew Alan Farmer et al. Jan 2005
US7045347 Advec, Inc. Frank L. Graham et al. May 2006
US7005277 Transgene S.A. Jean-Luc Imler et al. Feb 2006
US6468793 Florida State University Research Foundation John L. Teem Oct 2002
US6677155 The General Hospital Corporation Miguel Sena-Esteves et al. Jan 2004
US6696423 Biogen, Inc. James G. Barsoum et al. Feb 2004
US6649375 Genetic Theraphy, Inc. Sheila Connelly et al. Nov 2003
US6887856 University of Pittsburgh Joel S. Greenberger May 2005
US7129043 Duke University Rose-Mary N. Boustany et al. Oct 2006
US6974694 AdVec, Inc. Frank L. Graham et al. Dec 2005
US6482617 Aventis Pharma S.A. Patrice Yeh et al. Nov 2002
US6696272 HSC Research & Development Limited Partnership Don J. Mahuran et al. Feb 2004
US7105494 University of Pittsburgh of the Commonwealth System of Higher Education Axel W. Baltzer et al. Sep 2006
US7256181 Biogen Idec MA Inc. James G Barsoum et al. Aug 2007
US7135187 AdVec, Inc. Frank L. Graham et al. Nov 2006
US7037716 Crucell Holland B.V. Ronald Vogels et al. May 2006
US7314614 DNAVEC Research, Inc. Yoshikazu Yonemitsu et al. Jan 2008
US6716823 The UAB Research Foundation De-chu C. Tang et al. Apr 2004
US6602706 Introgene B.V. Frits Jacobus Fallaux et al. Aug 2003
US6878549 Introgene B.V. Ronald Vogels et al. Apr 2005
US6764674 Onyx Pharmaceuticals Inc. Terry Hermiston et al. Jul 2004
US6797265 Duke University Andrea Amalfitano et al. Sep 2004
US6783980 Crucell Holland B.V. Frits Jacobus Fallaux et al. Aug 2004
US7419808 Introgen Therapeutics, Inc. Shuyuan Zhang et al. Sep 2008
US7531523 Vertex Pharmaceuticals Incorporated Kenneth J. McCormack et al. May 2009
US7524510 The UAB Research Foundation John Jefferson Arnold et al. Apr 2009
US6358507 Genzyme Corporation Johanne Kaplan et al. Mar 2002
US6573090 The General Hospital Corporation Xandra O. Breakefield et al. Jun 2003
US6673602 The General Hospital Corporation Matthew A. Spear et al. Jan 2004
US6706693 The UAB Research Foundation De-chu Tang et al. Mar 2004
US6730507 Merck & Co., Inc. Frank L. Graham et al. May 2004
US7094399 Intronn, Inc. Edward Otto Aug 2006
US7063959 Beth Israel Deaconess Medical Center, Inc. Andrew Scharenberg Jun 2006
US7105346 Crucell Holland B.V. Frits J. Fallaux et al. Sep 2006
US7214515 The General Hospital Corporation E. Antonio Chiocca et al. May 2007
US6475480 Transgene S.A. Majid Mehtali et al. Nov 2002
US6692966 Crucell Holland B.V. Frits J. Fallaux et al. Feb 2004
US7144870 Research Development Foundation Saumya-Ray Chaudhuri et al. Dec 2006
US7445930 Introgen Therapeutics Inc. Shuyuan Zhang et al. Nov 2008
US7582442 The Regents of the University of Michigan James A. Shayman et al. Sep 2009
US7910545 Genzyme Corporation David Meeker et al. Mar 2011
US7666405 Duke University Andrea Amalfitano et al. Feb 2010
US7732197 The Penn State Research Foundation James S. Norris et al. Jun 2010
US7732129 Crucell Holland B.V. Shuyuan Zhang et al. Jun 2010
US7794707 University Health Network Joseph Penninger et al. Sep 2010
US7807457 TGT Laboratories, S.A. De C.V. Juan Armendariz Borunda et al. Oct 2010
US7855178 Vegenics Limited Kari Alitalo et al. Dec 2010
US7858368 TGT Laboratories, S.A. DE C.V. Juan Armendariz Borunda et al. Dec 2010
US6689605 UAB Research Foundation John D. Mountz et al. Feb 2004
US7422741 Vegenics Limited Kari Alitalo et al. Sep 2008
US7319015 The Regents of The University of Michigan James A. Shayman et al. Jan 2008
US6348450 The UAB Research Foundation De-chu C. Tang et al. Feb 2002
US6403370 GenStar Therapeutics Corporation Ramon Alemany et al. Jun 2002
US6566128 Merck & Co., Inc. Frank L. Graham et al. May 2003
US7575918 The Penn State Research Foundation James Norris et al. Aug 2009
US7915385 Vertex Pharmaceuticals Incorporated Kenneth John McCormack et al. Mar 2011
US7935342 Rinat Neuroscience Corp. John Chia-Yang Lin et al. May 2011

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