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US5689055: Plants having modified response to ethylene

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Filing Information

Inventor(s) Elliott M. Meyerowitz · Caren Chang · Anthony B. Bleecker ·
Assignee(s) California Institue of Technology ·
Attorney/Agent(s) Trecartin; Richard F.Flehr Hohbach Test Albritton & Herbert LLP ·
Primary Examiner Che S. Chereskin ·
Application Number US8530010
Filing date 09/19/1995
Issue date 11/18/1997
Predicted expiration date 07/01/2013
U.S. Classifications 800/205  · 800/DIG.44  ·
International Classifications A01H 400  ·
Kind CodeA
International Classifications 800205;DIG. 44;15 ·
Related U.S. Application DataThis is a continuation of application Ser. No. 08/086,555 filed Jul. 1, 1993 now abandoned.
8 Claims, No Drawings


Abstract

The invention includes transformed plants having at least one cell transformed with a modified ETR nucleic acid. Such plants have a phenotype characterized by a decrease in the response of at least one transformed plant cell to ethylene as compared to a plant not containing the transformed plant cell. Tissue and/or temporal specificity for expression of the modified ETR nucleic acid is controlled by selecting appropriate expression regulation sequences to target the location and/or time of expression of the transformed nucleic acid. The plants are made by transforming at least one plant cell with an appropriate modified ETR nucleic acid, regenerating plants from one or more of the transformed plant cells and selecting at least one plant having the desired phenotype.

Independent Claims | See all claims (8)

  1. 1. A tomato plant comprising plant cells transformed with a modified ETR nucleic acid comprising the modification of a codon of an ETR nucleic acid to substitute or delete an amino acid residue within the 313 N-terminal portion of the encoded ETR protein, wherein said ETR protein is at least 60% homologous and said N-terminal portion is at least 70% homologous to the ETR protein sequence of Arabidopsis thaliana as set forth in Seq. ID NO. 3, and wherein said tomato plant has a phenotype characterized by a decrease in response of said transformed plant cells to ethylene as compared to a wild-type tomato plant cells not containing said modified ETR nucleic acid.
  2. 4. A modified ETR nucleic acid comprising the modification of a codon of an ETR nucleic acid to substitute or delete an amino acid residue within the 313 amino acid N-terminal portion of the encoded ETR protein, wherein said ETR protein is at least 60% homologous and said N-terminal portion is at least 70% homologous to the ETR protein sequence of Arabidopsis thaliana as set forth in Seq. ID No. 3.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US4743548 Calgene, Inc. Crossway et al. May 1988
US4762785 Calgene, Inc. Comai Aug 1988
US4769061 Calgene Inc. Comai Sep 1988
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US5068193 Calgene, Inc. Comai Nov 1991
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Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
WO198912386Dec 1989
WO199001260Feb 1990
WO199101373Feb 1991
WO199101324Feb 1991
WO199211382Jul 1992
WO199212249Jul 1992
WO20WO9307264Apr 1993

Other Publications

"Ethylene in Plant Biology", 2d ed., F.B. Abeles, P.W. Morgan and M.E. Saltveit, Jr., eds. (San Diego), Academic Press, Inc. pp. 242-263, 1992.
Chang, et al. (1992 Feb.) Biochemical Society Transactions 20(1): 73-75.
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Oeller, P.W. et al. (1991) Science 254:437-439, "Reversible Inhibition of Tomato Fruit Senescence by Antisense RNA".
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Chang et al., "Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana" PNAS USA, 85:6856-6860 (1988).
Pickett et al., "Recessive Mutation at the ETR-2 Locus of Arabidopsis thaliana Confers Resistance to Some Effects of Ethylene Exposure," J. Cell. Biochem., Supp. 0 (13 part D):324 (1989). Symposium on Plant Gene Transfer, 18th Annual UCLA Symposium, Park City, Utah, USA: Apr. 1-7, 1989.
Boswell et al., "Computational Molecular Biology Sources and Methods for Sequence Analysis," (Lest, ed.) Oxford University Press, Oxford 1989, pp. 170-171.
Chang et al., "Arabidopsis Ethylene-Response Gene ETR1: Similarity of Product of Two-Component Regulators," Science, 262:539-544 (1993).
Chang et al., "Eukaryotes have two-component Signal Transducers," Res. Microb., 1459:481-486 (1994).
Lawton et al., "Acquired-Resistance Signal-Transduction in Arabidopsis is Ethylene Independent," Cell, 6(5):581-588 (1994).
Chang, C., et al., "The TMKI Gene from Arabidopsis Codes for a Protein with Structural and Biochemical Characteristics of a Receptor Protein Kinase," Plant Cell, 4:1263 (1992).
Bleeker, A. B., "Genetic Analysis of Ethylene Responses in Arabidopsis thaliana," Great Britain Society for Experimental Biology (1991).

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