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US5797871: Ultrasonic cleaning of allograft bone

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Filing Information

Inventor(s) Wolfinbarger, Jr.; Lloyd ·
Assignee(s) LifeNet Research Foundation ·
Attorney/Agent(s) Jones & Volentine, L.L.P. ·
Primary Examiner Michael Buiz ·
Assistant Examiner Deborah Blyvers ·
Application Number US8646519
Filing date 05/07/1996
Issue date 08/25/1998
Predicted expiration date 08/19/2014
U.S. Classifications 604/49  · 128/898  ·
International Classifications A61M 3100  ·
Kind CodeA
International Classifications 128898 ·
Related U.S. Application DataBACKGROUND OF THE INVENTION
This application is a continuation-in-part of co-pending U.S. application Ser. No. 08/646,520 filed May 7, 1996 entitled "A Recirculation Method for Cleaning Essentially Intact Bone Grafts Using Pressure Medicated flow of Solvents and Bone Grafts Produced Thereby", and this application is a Continuation-in-Part of U.S. application Ser. No. 08/395,113 filed Feb. 27, 1995 now U.S. Pat. No. 5,556,379 which is a Continuation-in-Part of U.S. application Ser. No. 08/293,206 filed Aug. 19, 1994, now abandoned, all of which we incorporated by reference herein in their entirety.
25 Claims, No Drawings


Abstract

The present invention is directed to a method for cleaning cadaveric donor bone to produce bone grafts suitable for transplantation into a human, as well as the bone grafts produced thereby. The present method involves removing bone marrow potentially containing bacteria, virus or fungi, from the donor bone by sonicating the bone in a solvent containing one or more detergents to produce bone grafts essentially free from bone marrow, as well as bone grafts essentially free from any detectable bacterial, fungal, or viral contamination.

Independent Claims | See all claims (25)

  1. 1. A method for producing a bone graft suitable for transplantation into a human, comprising:sonicating said bone graft with a solvent comprising one or more detergents in an ultrasonic cleaner at a temperature from 37.degree. to 50.degree. C. and for a time period effective to produce a cleaned bone graft essentially free from bone marrow.
  2. 10. A method for producing a bone graft suitable for transplantation into a human, comprising:sonicating at a temperature from 37.degree. to 50.degree. C. said bone graft using an ultrasonic cleaning device with a first solvent comprising one or more detergents to produce a first cleaned bone graft, andwherein said first cleaned bone graft is essentially free from bone marrow.
  3. 22. A method for producing an essentially intact bone graft suitable for transplantation into a human, comprising:inducing a negative pressure mediated flow of a first solvent, said first solvent comprising one or more detergents, through an opening in a bone shaft of said essentially intact bone graft to produce a cleaned intact bone graft;sonicating said essentially intact bone graft in a container with said first solvent using an ultrasonic cleaner,wherein said inducing and said sonicating are carried out simultaneously for a time effective to produce a cleaned intact bone graft essentially free from bone marrow.
  4. 23. A method for producing an essentially intact bone graft suitable for transplantation into a human, comprising:sonicating said bone graft with a solvent comprising one or more detergents in an ultrasonic cleaner at a temperature and for a time period effective to produce a cleaned bone graft essentially free from bone marrow and,inducing a pressure mediated flow of said solvent through an opening in a bone shaft of said essentially intact bone graft wherein said pressure mediated flow is carried out for a period of time effective to produce a cleaned bone graft essentially free from bone marrow, andwherein said step of sonicating and said step of inducing are carried out simultaneously.
  5. 24. A method for producing a bone graft suitable for transplantation into a human, comprising:sonicating said bone graft using an ultrasonic cleaning device with a first solvent comprising one or more detergents to produce a first cleaned bone graft;sonicating said first cleaned bone graft using an ultrasonic cleaning device with a second solvent comprising one or more members selected form the group consisting of: an antibiotic, an antimycotic and an antiviral agent, to produce a second cleaned bone graft, andsonicating said second cleaned bone graft using an ultrasonic cleaning device with a third solvent comprising one or more decontaminating agents to produce a third cleaned bone graft, wherein said third cleaned bone graft is essentially free from bone marrow.
  6. 25.25. A method for producing a bone graft suitable for transplantation into a human, comprising:sonicating said bone graft using an ultrasonic cleaning device with a first solvent comprising one or more detergents to produce a first cleaned bone graft;agitating said first cleaned bone graft with a second solvent comprising one or more members selected from the group consisting of: an antibiotic, an antimycotic and an antiviral agent, to produce a second cleaned bone graft; andagitating said second cleaned bone graft with a third solvent comprising one or more decontaminating agents to produce a third cleaned bone graft, wherein said third cleaned bone graft is essentially free from bone marrow.

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Referenced By

Document NumberAssigneeInventorsIssue/Pub Date
AU770935 REGENERATION TECH INC MILLS C RANDAL et al. Mar 2004
FR2866237 BIOBANK BARDONNET RAPHAEL Aug 2005
US6143033 Synthes (USA) David C. Paul et al. Nov 2000
US6258125 Synthes (U.S.A.) David C. Paul et al. Jul 2001
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