Enzyme dispersions, their production and compositions containing them
Filing Information
- Patent Number: US5801140
- Application Number: US8545603
- Filing date: 02/07/1996
- PCT 371 date: 02/07/1996
- Issue date: 09/01/1998
- Predicted expiration date: 02/07/2016
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analyzing 100 million+ documents to uncover your network...- U.S. Classifications: 510/530 · 435/188 ·
- International Classifications: C11D 3386 ·
- International Classifications: 510392;393;421;434;408;409;415;417;418;530 ·
- PCT Application Number: PCTGB9400960 - 05/05/1994
- PCT Publication Number: WO199425560 - 11/10/1994
- Foreign Priority: GB9309243 - 05/05/1993 ·
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USPTO · PAIR · esp@cenet · Patent Family* Related patent documents may or may not exist on these sites
Abstract
A process for the production of stable fluid enzyme dispersions having a content of at least 0.5% by weight enzyme, in which the enzyme is present in the form of anhydrous particles of a diameter less than 30 .mu.m, comprising emulsifying an enzyme composition into a water immiscible liquid in the presence of a polymeric dispersion stabilizer to form a stable dispersion of aqueous enzyme particles having a dry size below 30 .mu.m dispersed in the liquid, and dehydrating the dispersed particles by azeotropic distillation, wherein before, during or after dehydrating the particles the process comprises adding an organic liquid which is less volatile than the water immiscible liquid and which is selected from surfactants and water miscible liquids to the dispersion and distilling the water immiscible liquid from the dispersion until the amount of the initial water immiscible liquid remaining in the dispersion is below 20% by weight of the liquid phase in the dispersion.References Cited
U.S. Patent Documents
| Document Number | Assignees | Inventors | Issue/Pub Date |
|---|---|---|---|
| US4115292 | The Procter & Gamble Company | Richardson et al. | Sep 1978 |
| US5324445 | Allied Colloids Limited | Langley et al. | Jun 1994 |
| US5460817 | Allied Colloids Ltd. | Langley et al. | Oct 1995 |
| US5492646 | Allied Colloids Limited | Langley et al. | Feb 1996 |
| US5589370 | Lever Brothers Company, Division of Conopco, Inc. | Ratuiste et al. | Dec 1996 |
Referenced By
| Document Number | Assignee | Inventors | Issue/Pub Date |
|---|---|---|---|
| US6673763 | Novozymes A/S | Ole Regnar Hansen et al. | Jan 2004 |
| US7601858 | GS Cleantech Corporation | David Fred Cantrell et al. | Oct 2009 |
| US7608729 | GS Cleantech Corporation | David J. Winsness et al. | Oct 2009 |
| US6242405 | Novo Nordisk A/S | Mads Lykke et al. | Jun 2001 |
| US6221829 | Novo Nordisk A/S | Kenneth Charles Symes et al. | Apr 2001 |
Patent Family
| Document Number | Assignee | Inventors | Issue/Pub Date |
|---|---|---|---|
| GB9309243 | ALLIED COLLOIDS LTD | Jun 1993 | |
| WO199425560 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | Nov 1994 |
| CA2162118 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | Nov 1994 |
| AU6682094 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | Nov 1994 |
| MX9403376 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | Jan 1995 |
| ZA9403117 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | May 1995 |
| FI955234 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | Nov 1995 |
| NO954418 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | Nov 1995 |
| JP8509761 | Oct 1996 | ||
| AU687395 | ALLIED COLLOIDS LTD | LANGLEY JOHN GRAHAM et al. | Feb 1998 |
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Independent Claims | See all claims (22)
- 1. A process for making a stable fluid dispersion of an enzyme or composition meltable to form such a dispersion comprising emulsifying an enzyme composition into water immiscible liquid in the presence of a polymeric dispersion stabiliser to form a stable dispersion of aqueous enzyme particles having a dry size below 30 .mu.m dispersed in the liquid, and dehydrating the dispersed particles by azeotropic distillation, and in which before, during or after dehydrating the particles an organic liquid which is less volatile than the water immiscible liquid and which is selected from surfactants and water miscible liquids is then added to the dispersion and the water immiscible liquid is distilled from the dispersion until the amount of the initial water immiscible liquid remaining in the dispersion is below 20% by weight of the liquid phase in the dispersion.
- 4. A process for making a substantially anhydrous stable fluid dispersion of an enzyme or composition meltable to form such a dispersion comprising emulsifying an enzyme composition into water immiscible liquid in the presence of a polymeric dispersion stabilizer to form a stable dispersion of aqueous enzyme particles having a dry size below 30 .mu.m dispersed in the liquid, and dehydrating the dispersed particles by azeotropic distillation, and in which before, during or after dehydrating the particles an organic liquid which is less volatile than the water immiscible liquid and which is surfactant added to the dispersion and the water immiscible liquid is then distilled from the dispersion until the amount of the initial water immiscible liquid remaining in the dispersion is below 20% by weight of the liquid phase in the dispersion and at least 80% by weight of the liquid phase in the dispersion produced is surfactant.
- 11. A stable fluid dispersion in a non-aqueous liquid phase of anhydrous particles of enzyme or a composition meltable to form such a dispersion wherein the particles are below about 30 .mu.m in size, the dispersion is stabilised by the inclusion of polymeric dispersion stabiliser, the amount of enzyme (on an active weight basis) is at least about 0.5% by weight of the dispersion, the amount of liquid hydrocarbon in the liquid phase is below 20% by weight of the liquid phase, and more than 80% by weight of the liquid phase is selected from surfactants and water miscible liquids.
