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US5824868: Plants having modified response to ethylene


Filing Information

Inventor(s) Elliot M. Meyerowitz · Caren Chang · Anthony B. Bleecker ·
Assignee(s) California Institute of Technology ·
Attorney/Agent(s) Flehr Hohbach Test Albritton & Herbert LLP ·
Primary Examiner David T. Fox ·
Assistant Examiner Amy J. Nelson ·
Application Number US8484101
Filing date 06/07/1995
Issue date 10/20/1998
Predicted expiration date 07/01/2013
U.S. Classifications 800/205  · 435/172.3  ·
International Classifications A01H 500  ·
Kind CodeA
International Classifications 43569.1;320.1;172.3;240.4;419 ·
Related U.S. Application DataThis a continuation-in-part of U.S. application Ser. No. 08/263,480 filed Jun. 28, 1994, now abandoned, which in turn is a continuation-in-part of U.S. application Ser. No. 08/086,555 filed Jul. 1, 1993, now abandoned.
14 Claims, No Drawings


The invention includes transformed plants having at least one cell transformed with a modified ETR nucleic acid. Such plants have a phenotype characterized by a decrease in the response of at least one transformed plant cell to ethylene as compared to a plant not containing the transformed plant cell. Tissue and/or temporal specificity for expression of the modified ETR nucleic acid is controlled by selecting appropriate expression regulation sequences to target the location and/or time of expression of the transformed nucleic acid. The plants are made by transforming at least one plant cell with an appropriate modified ETR nucleic acid, regenerating plants from one or more of the transformed plant cells and selecting at least one plant having the desired phenotype.

Independent Claims | See all claims (14)

  1. 1. An antisense expression construct comprising a promoter and an ETR nucleic acid in the antisense orientation, wherein said ETR nucleic acid encodes an ETR protein with at least 50% overall similarity to the ETR protein sequence of Arabidopsis thaliana as set forth in SEQ ID NO:3 and at least 60% similarity to the N-terminal 316 amino acids of SEQ ID NO:3, and wherein expression of said construct in a plant cell results in a decreased response to ethylene by said cell.
  2. 2. An antisense expression construct comprising a promoter and an ETR nucleic acid in the antisense orientation, wherein said ETR nucleic acid hybridizes to the nucleic acid sequence of SEQ ID NO:2 at hybridization conditions of C. in 5.times. SSPE and wash conditions of C. in 0.2.times. SSPE, and wherein expression of said construct in a plant cell results in a decreased response to ethylene by said cell.

References Cited

U.S. Patent Documents

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US4762785 Calgene, Inc. Comai Aug 1988
US4769061 Calgene Inc. Comai Sep 1988
US4801540 Calgene, Inc. Hiatt et al. Jan 1989
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US4956282 Calgene, Inc. Goodman Sep 1990
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US5107065 Calgene, Inc. Shewmaker et al. Apr 1992
US5110728 Calgene, Inc. Kridl et al. May 1992
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US5175095 Calgene, Inc. Martineau et al. Dec 1992
US5177011 Calgene, Inc. Shewmaker et al. Jan 1993
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Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
WO198912386Dec 1989
WO199001260Feb 1990
WO199101373Feb 1991
WO199101324Feb 1991
WO199211382Jul 1992
WO199212249Jul 1992
WO199307264Apr 1993

Other Publications

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