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US6287761: Synthetic antigens for the detection of antibodies to hepatitis C virus

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Filing Information

Inventor(s) Robert J. Deleys · Dirk Pollet · Geert Maertens · Hugo Van Heuverswijn ·
Assignee(s) Innogenetics N.V. ·
Attorney/Agent(s) Nixon & Vanderhye P.C. ·
Primary Examiner Donna C. Wortman ·
Application Number US9275265
Filing date 03/23/1999
Issue date 09/11/2001
Predicted expiration date 02/21/2015
U.S. Classifications 435/5  · 530/326  · 436/820  · 530/327  · 436/518  ·
International Classifications --
Kind CodeB1
International Classifications 435 5 · 530326 · 530327 · 436518 · 436820 ·
Related U.S. Application DataThis application is a continuation of application No. 08/391,671, filed Feb. 21, 1995, now U.S. Pat. No. 5,922,352, which is a continuation of application No. 07/920,286, filed Oct. 14, 1992, abandoned, which is a 371 of PCT/EP91/02409, filed Dec. 31, 1991.
Foreign Priority EP90124241 - 12/14/1990 ·
28 Claims, 8 Drawings


Abstract

Peptide sequences are provided which are capable of mimicking proteins encoded by HCV for use as reagents for screening of blood and blood products for prior exposure to HCV. The peptides are at least 5 amino acids long and can be used in various specific assays for the detection of antibodies to HCV, for the detection of HCV antigens, or as immunogens.

Independent Claims | See all claims (28)

  1. 1. A peptide consisting of at least 5 to less than 20 amino acids located in the region consisting of amino acids 1688 to 1707 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV.
  2. 5. A peptide consisting of at least 5 to less than 20 amino acids located in the region consisting of amino acids 1694 to 1713 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV.
  3. 9. A peptide consisting of at least 5 to less than 20 amino acids located in the region consisting of amino acids 1706 to 1725 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV.
  4. 13. A peptide consisting of at least 5 to less than 20 amino acids located in the region consisting of amino acids 1712 to 1731 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV.
  5. 17. A peptide consisting of at least 5 to at most 12 amino acids located in the region consisting of amino acids 1718 to 1737 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV.
  6. 19. A peptide consisting of at least 5 to less than 20 amino acids located in the region consisting of amino acids 1724 to 1743 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV.
  7. 23. A peptide consisting of at least 5 to at most 12 amino acids located in the region consisting of amino acids 1730 to 1749 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV.
  8. 25. A method for the detection of antibodies to hepatitis C virus present in a body fluid comprising the steps of: (a) contacting a body fluid of a person to be diagnosed with a peptide consisting of at least 5 to less than 20 amino acids located in the region consisting of amino acids 1718 to 1737 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV, and, (b) detecting an immunological complex formed between antibodies in said body fluid and said peptide as an indication of the presence of antibodies to hepatitis C virus.
  9. 26. A method for the detection of antibodies to hepatitis C virus present in a body fluid comprising the steps of: (a) contacting a body fluid of a person to be diagnosed with a peptide consisting of at least 5 to less than 20 amino acids located in the region consisting of amino acids 1730 to 1749 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV, and, (b) detecting an immunological complex formed between antibodies in said body fluid and said peptide as an indication of the presence of antibodies to hepatitis C virus.
  10. 27. A kit for the detection of anti-hepatitis C virus antibodies in a body fluid, comprising: a peptide consisting of at least 5 to at most 12 amino acids located in the region consisting of amino acids 1718 to 1737 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV, and a means for detecting an immunological complex formed between said peptide and said antibodies.
  11. 28. A kit for the detection of anti-hepatitis C virus antibodies in a body fluid, comprising: a peptide consisting of at least 5 to at most 12 amino acids located in the region consisting of amino acids 1730 to 1749 of the HCV polyprotein of an HCV isolate which is capable of providing for immunological competition with at least one strain of HCV, and a means for detecting an immunological complex formed between said peptide and said antibodies.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US5106726 United Biomedical, Inc. Wang Apr 1992
US5302507 Japan as represented by Director General of the Agency of the National Institute of Health Chiba et al. Apr 1994
US5350671* Chiron Corporation Houghton et al. Sep 1994
US5910404 Innogenetics N.V. Deleys Jun 1999

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
EP0318216CHIRON CORPORATIONMay 1989
EP388232Sep 1990
EP0442394United Biomedical, Inc.Aug 1991
EP0445423ABBOTT LABORATORIESSep 1991
EP0445801Kuraray Co., Ltd.Sep 1991
EP0451891Akzo Nobel N.V.Oct 1991
EP0450931CHIRON CORPORATIONOct 1991
EP0468527United Biomedical, Inc.Jan 1992
EP0471356JAPAN as represented by Director General of Agency of NATIONAL INSTITUTE OF HEALTHFeb 1992
EP0484787Dade Behring Marburg GmbHMay 1992
WO198904669Jun 1989
WO199201714Feb 1992
* cited by examiner

Other Publications

Viancks, R., Eur. J. Clin. Microbiol. Infec. Dis., 9(9), 1990 “Evaluation of a line immunoassay for the differential detection of antibodies of human immunodeficiency virus”, 674-676.
Shimonishi Y., Ed. Peptide Chemistry, 1990 “Proceedings of the 26th Symposium on Peptide Chemistry”, Osaka, Oct. 25-27, 1990.
Munekata et al., “Eptitope-Mapping of Hepatitis C Virus Constituting Protein” pp. 211-214, Protein Research Foundation, Osaka, 1991.
Choo, Q.L., et al,
Kuo, G., et al,
Okamoto, H., et al,
Okamoto, H., et al,

Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
US5910404 Innogenetics N.V. Robert J. Deleys et al. Jun 1999
US6287761 Innogenetics N.V. Robert J. Deleys et al. Sep 2001