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US6294716: Plants having modified response to ethylene by transformation with an ETR nucleic acid

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Filing Information

Inventor(s) Elliott M. Meyerowitz · Caren Chang · Anthony B. Bleecker ·
Assignee(s) California Institute of Technology ·
Attorney/Agent(s) Richard F. Trecartin · Flehr Hohbach Test Albritton & Herbert LLP ·
Primary Examiner Amy J. Nelson ·
Application Number US8714524
Filing date 09/16/1996
Issue date 09/25/2001
Predicted expiration date 07/01/2013
U.S. Classifications 800/317.4  · 536/236  · 435/440  · 435/419  · 800/283  · 800/298  · 435/320.1  ·
International Classifications --
Kind CodeB1
International Classifications 536 236 · 4351723 · 4353201 · 435419 · 435 691 · 435468 · 435440 · 435283 · 800205 · 800DIG 15 · 800DIG 44 · 800 691 · 800278 · 800298 · 8003174 · 800287 ·
Related U.S. Application DataThis is a continuation of application Ser. No. 08/263,480 filed Jun. 28, 1994, now abandoned which is a continuation-in-part of application Ser. No. 08/086,555 filed Jul. 1, 1993, now abandoned.
32 Claims, 65 Drawings


Abstract

The invention includes transformed plants having at least one cell transformed with a modified ETR nucleic acid. Such plants have a phenotype characterized by a decrease in the response of at least one transformed plant cell to ethylene as compared to a plant not containing the transformed plant cell. Tissue and/or temporal specificity for expression of the modified ETR nucleic acid is controlled by selecting appropriate expression regulation sequences to target the location and/or time of expression of the transformed nucleic acid. The plants are made by transforming at least one plant cell with an appropriate modified ETR nucleic acid, regenerating plants from one or more of the transformed plant cells and selecting at least one plant having the desired phenotype.

Independent Claims | See all claims (32)

  1. 1. An isolated nucleic acid comprising a plant ETR nucleic acid encoding an ETR protein, said ETR protein having at least 50% overall similarity to the ETR protein sequence of Arabidopsis thaliana as set forth in SEQ ID NO:3, and at least 55% similarity to the N-terminal 316 amino acids of said ETR protein sequence of Arabidopsis thaliana, wherein the expression of said ETR protein encoded by said ETR nucleic acid in a plant cell results in an increased or decreased response to ethylene by said cell.
  2. 4. An isolated nucleic acid comprising a plant ETR nucleic acid encoding an ETR protein, said ETR protein having at least 50% overall similarity to the ETR protein sequence of Arabidopsis thaliana as set forth in SEQ ID NO:3, and at least 55% similarity to the N-terminal 316 amino acids of said ETR protein sequence of Arabidopsis thaliana, wherein the expression of said ETR protein encoded by said ETR nucleic acid in a plant cell results in an increased response to ethylene by said cell.
  3. 5. An isolated modified plant ETR nucleic acid comprising a precursor ETR nucleic acid which has been modified to encode a modified ETR protein comprising the substitution, insertion or deletion of an amino acid residue in the N-terminal 316 amino acids of the ETR protein encoded by said precursor ETR nucleic acid, wherein said modified ETR protein has at least 50% overall similarity to the ETR protein sequence of Arabidopsis thaliana as set forth in SEQ ID NO:3, and at least 55% similarity to the N-terminal 316 amino acids of said ETR protein sequence of Arabidopsis thaliana, wherein the expression of said modified ETR protein encoded by said isolated modified plant ETR nucleic acid in a plant cell results in an increased or decreased response to ethylene by said cell.
  4. 9. An isolated modified plant ETR nucleic acid comprising a precursor ETR nucleic acid which has been modified to encode a modified ETR protein comprising the substitution or deletion of an amino acid residue in the N-terminal 316 amino acids of the ETR protein encoded by said precursor ETR nucleic acid, wherein said modified ETR protein has at least 50% overall similarity to the ETR protein sequence of Arabidopsis thaliana as set forth in SEQ ID NO:3, and at least 55% similarity to the N-terminal 316 amino acids of said ETR protein sequence of Arabidopsis thaliana, wherein the expression of said modified ETR protein encoded by said isolated modified plant ETR nucleic acid in a plant cell results in a decreased response to ethylene by said cell.
  5. 16. A recombinant nucleic acid comprising a promoter operably linked to an ETR nucleic acid, wherein said ETR nucleic acid hybridizes with a probe having the sequence represented in SEQ ID NO:2 at hybridization conditions of 50° C. in 5×SSPE and washing conditions of 50° C. in 0.2×SSPE, wherein the expression of an ETR protein encoded by said ETR nucleic acid in a plant cell results in an increased or decreased response to ethylene by said cell.
  6. 17. A method of producing a plant comprising transformed plant cells having a detectable increased or decreased response to ethylene as compared to untransformed cells of a corresponding wild-type plant, said method comprising the steps of: a) transforming at least one plant cell with a modified ETR nucleic acid comprising a precursor ETR nucleic acid which has been modified to encode a modified ETR protein comprising the substitution, insertion or deletion of an amino acid residue in the N-terminal 316 amino acids of the ETR protein encoded by said precursor ETR nucleic acid, wherein said modified ETR protein has at least about 50% overall similarity to the ETR protein sequence of Arabidopsis thaliana as set forth in SEQ ID NO:3, and at least about 55% similarity to the N-terminal 316 amino acids of said ETR protein sequence of Arabidopsis thaliana; b) regenerating plants from one or more of the thus transformed plant cells; and c) selecting a plant comprising said transformed plant cells having a detectable increased or decreased response to ethylene.
  7. 19. A method of producing a plant comprising transformed plant cells having a detectable decrease in response to ethylene as compared to untransformed cells of a corresponding wild-type plant, said method comprising the steps of: a) transforming at least one plant cell with a modified ETR nucleic acid comprising a precursor ETR nucleic acid which has been modified to encode a modified ETR protein comprising a substitution, insertion or deletion of an amino acid residue in the N-terminal 316 amino acids of the ETR protein encoded by said precursor ETR nucleic acid, wherein said modified ETR protein has at least about 50% overall similarity to the ETR protein sequence of Arabidopsis thaliana as set forth in SEQ ID NO:3, and at least about 55% similarity to the N-terminal 316 amino acids of said ETR protein sequence of Arabidopsis thaliana; b) regenerating plants from one or more of the thus transformed plant cells; and c) selecting a plant comprising said transformed plant cells having a detectable decrease in response to ethylene.

References Cited

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Other Publications

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