Filing Information

Inventor(s)	Imre Kovesdi · Duncan L. McVey ·
Assignee(s)	GenVec, Inc. ·  View assignee updates
Attorney/Agent(s)	Leydig, Voit & Mayer, Ltd. ·
Primary Examiner	James Housel ·
Assistant Examiner	Bao Qun Li ·
Application Number	US9685914
Filing date	10/10/2000
Issue date	06/03/2003
Predicted expiration date	10/25/2020
Patent term adjustment	15
U.S. Classifications	435/320.1  · 435/325  · 435/914.1  · 435/914.2  · 435/914  · 435/913.2  · 424/199.1  · 424/205.1  · 424/233.1  · 435/913.3  ·
International Classifications	--
Kind Code	B1
International Classifications	4353201 · 435325 · 435 9133 · 435 9132 · 435 9141 · 435 9142 · 424 914 · 4241991 · 4242051 · 4242331 ·

Patent Family

Abstract

The inventive method of producing a eukaryotic viral vector comprises contacting a eukaryotic cell, which comprises a unique enzyme that nicks or cleaves a DNA molecule, with a recombinant phage vector, or contacting a eukaryotic cell, which does not comprise a unique enzyme that nicks or cleaves a DNA molecule, simultaneously or sequentially, in either order, with (i) a unique enzyme that nicks or cleaves a DNA molecule, and (ii) a recombinant phage vector. The recombinant phage vector comprises the DNA molecule comprising (a) a eukaryotic viral vector genome comprising a coding sequence, (b) a phage packaging site that is not contained within the eukaryotic viral vector genome, and (c) a promoter that is operably linked to the coding sequence. The DNA molecule enters the eukaryotic cell, and the unique enzyme nicks or cleaves the DNA molecule in the eukaryotic cell in at least one region not contained within the eukaryotic viral vector genome, thereby inducing the production of and ultimately producing a eukaryotic viral vector.

Independent Claims | See all claims (21)

1. 1. A method of producing a eukaryotic viral vector, which method comprises: contacting a eukaryotic cell, which comprises a genorne and which does not comprise a unique enzyme that nicks or cleaves a DNA molecule, simultaneously or sequentially, in either order, with (i) a unique enzyme that nicks or cleaves a DNA molecule and (ii) a recombinant phage vector, wherein the recombinant phage vector comprises the DNA molecule comprising (a) a eukaryotic viral vector genome comprising a coding sequence, (b) a phage packaging site and a unique enzyme site that are not contained within the eukaryotic viral vector genome, and (c) a promoter that is operably linked to the coding sequence, whereupon the DNA molecule enters the eukaryotic cell and the unique enzyme nicks or cleaves the unique enzyme site in the DNA molecule in the eukaryotic cell, whereupon a eukaryotic viral vector is produced.