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US6573092: Method of preparing a eukaryotic viral vector

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Filing Information

Inventor(s) Imre Kovesdi · Duncan L. McVey ·
Assignee(s) GenVec, Inc. ·
Attorney/Agent(s) Leydig, Voit & Mayer, Ltd. ·
Primary Examiner James Housel ·
Assistant Examiner Bao Qun Li ·
Application Number US9685914
Filing date 10/10/2000
Issue date 06/03/2003
Predicted expiration date 10/25/2020
Patent term adjustment 15
U.S. Classifications 435/320.1  · 435/325  · 435/914.1  · 435/914.2  · 435/914  · 435/913.2  · 424/199.1  · 424/205.1  · 424/233.1  · 435/913.3  ·
International Classifications --
Kind CodeB1
International Classifications 4353201 · 435325 · 435 9133 · 435 9132 · 435 9141 · 435 9142 · 424 914 · 4241991 · 4242051 · 4242331 ·
21 Claims, No Drawings


Abstract

The inventive method of producing a eukaryotic viral vector comprises contacting a eukaryotic cell, which comprises a unique enzyme that nicks or cleaves a DNA molecule, with a recombinant phage vector, or contacting a eukaryotic cell, which does not comprise a unique enzyme that nicks or cleaves a DNA molecule, simultaneously or sequentially, in either order, with (i) a unique enzyme that nicks or cleaves a DNA molecule, and (ii) a recombinant phage vector. The recombinant phage vector comprises the DNA molecule comprising (a) a eukaryotic viral vector genome comprising a coding sequence, (b) a phage packaging site that is not contained within the eukaryotic viral vector genome, and (c) a promoter that is operably linked to the coding sequence. The DNA molecule enters the eukaryotic cell, and the unique enzyme nicks or cleaves the DNA molecule in the eukaryotic cell in at least one region not contained within the eukaryotic viral vector genome, thereby inducing the production of and ultimately producing a eukaryotic viral vector.

Independent Claims | See all claims (21)

  1. 1. A method of producing a eukaryotic viral vector, which method comprises: contacting a eukaryotic cell, which comprises a genorne and which does not comprise a unique enzyme that nicks or cleaves a DNA molecule, simultaneously or sequentially, in either order, with (i) a unique enzyme that nicks or cleaves a DNA molecule and (ii) a recombinant phage vector, wherein the recombinant phage vector comprises the DNA molecule comprising (a) a eukaryotic viral vector genome comprising a coding sequence, (b) a phage packaging site and a unique enzyme site that are not contained within the eukaryotic viral vector genome, and (c) a promoter that is operably linked to the coding sequence, whereupon the DNA molecule enters the eukaryotic cell and the unique enzyme nicks or cleaves the unique enzyme site in the DNA molecule in the eukaryotic cell, whereupon a eukaryotic viral vector is produced.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US5559099 GenVec, Inc. Wickham et al. Sep 1996
US5712136 GenVec, Inc. Wickham et al. Jan 1998
US5736388 Chada et al. Apr 1998
US5846782 GenVec, Inc. Wickham et al. Dec 1998
US5851806 GenVec, Inc. Kovesdi et al. Dec 1998
US5962311 GenVec, Inc. Wickham et al. Oct 1999
US5994106 GenVec, Inc. Kovesdi et al. Nov 1999

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
WO199534671Dec 1995
WO199625506Aug 1996
WO199700326Jan 1997
WO199720051Jun 1997
WO199721826Jun 1997
WO199856937Dec 1998
WO199902647Jan 1999
WO199911764Mar 1999
WO199915683Apr 1999
WO199915684OXFORD BIOMEDICA (UK) LIMITEDApr 1999
WO200014269AMIRA MEDICALMar 2000

Other Publications

Miyake et al. P.N.A.S. 1996, vol. 93, pp. 1320-1324.*
Brough et al.,
Castro-Peralta et al.,
Horwitz, “Adenoviruses,”
Kojima et al.,

Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
AU1313302 GENVEC INC KOVESDI IMRE et al. Apr 2002
US6573092 GenVec, Inc. Imre Kovesdi et al. Jun 2003
US20030203480 GenVec, Inc. Duncan McVey et al. Oct 2003
US6908762 GenVec, Inc. Imre Kovesdi et al. Jun 2005