Filing Information

Inventor(s)	Robert Ziegelmaier ·
Assignee(s)	Dade Behring Marburg GmbH ·  View assignee updates
Attorney/Agent(s)	Finnegan, Henderson, Farabow, Garrett and Dunner, L.L.P. ·
Primary Examiner	Bao Thuy L. Nguyen ·
Application Number	US8445584
Filing date	05/22/1995
Issue date	10/14/2003
Predicted expiration date	05/20/2008
U.S. Classifications	436/513  · 436/820  · 436/523  · 436/538  · 436/548  · 436/518  · 435/772  · 435/79  · 435/794  · 435/962  · 436/512  ·
International Classifications	--
Kind Code	B1
International Classifications	436518 · 436523 · 436538 · 436548 · 436513 · 436512 · 436820 · 435 772 · 435 79 · 435 794 · 435962 ·
Related U.S. Application Data	This application is a continuation application filed May 22, 1995 of parent application U.S. Ser. No. 07/988,007, filed Dec. 9, 1992, now abandoned, which is a continuation application of Ser. No. 07/670,523 filed Mar. 18, 1991, now abandoned, which is a continuation application of Ser. No. 07/434,472 filed Nov. 14, 1989, now abandoned, which is a continuation application of Ser. No. 07/196,526 filed May 20, 1988, now abandoned.
Foreign Priority	DE3717401 - 05/23/1987 ·

Patent Family

Abstract

An immunochemical method for the determination of antibodies which are specific for an antigen and are of one of the immunoglobulin classes: A, M, D or E in a fluid, with this fluid being contacted with a solid phase to which the antibodies against this immunoglobulin class, or a fragment of an antibody of this type, are bound, which results in the immunoglobulin of this class being bound to this solid phase, and this solid phase being contacted with the antigen, which carries a labeling means where appropriate, and with a labeled antibody or a labeled fragment of an antibody against the antigen if the antigen is unlabeled and determination, from the amount of labeling means which is bound to the solid phase, of the amount of these antibodies which are specific for an antigen and are one of the immunoglobulin classes, which comprises the solid phase being simultaneously in contact with the fluid containing the antibody which is to be determined and with the antigen, which is labeled where appropriate, there being addition of a substance which prevents immunoglobulin G binding to the solid phase and, where appropriate, antigen binding to immunoglobulin G, and an agent suitable for this purpose, are described.

Independent Claims | See all claims (16)

1. 1. An immunological method for the detection of an antigen specific antibody comprising one or more of the immunoglobulin classes A, M, D, or E in a fluid, comprising the simultaneous incubation of: a. a solid phase having bonded thereto an antibody specific for said immunoglobulin classes A, M, D, or E; b. a fluid containing an immunoglobulin of classes A, M, D, or E; c. an unlabeled antigen immunologically reactive to the fluid phase immunoglobulin; d. a labeled antibody, immunologically reactive with said unlabeled antigen; and e. a substance which inhibits the binding of immunoglobulin G to the solid phase and which inhibits the binding of said unlabeled antigen to immunoglobulin G.
2. 9. An immunological method for the detection of an antigen specific antibody comprising one or more of the immunoglobulin classes A, M, D or E in a fluid, comprising the simultaneous incubation of: a. a solid phase having bonded thereto an antibody specific for said immunoglobulin classes A, M, D, or E; b. a fluid containing an immunoglobulin of classes A, M, D, or E; c. a labeled antigen immunologically reactive to the fluid phase immunoglobulin; and d. a substance which inhibits the binding of immunoglobulin G to the solid phase and which inhibits the binding of said labeled antigen to immunoglobulin G.