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US6632682: One-step immunoassay for the determination of antigen-specific antibodies of one of the immunoglobulin classes A, M, D, or E, and an agent suitable for this purpose

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Filing Information

Inventor(s) Robert Ziegelmaier ·
Assignee(s) Dade Behring Marburg GmbH ·
Attorney/Agent(s) Finnegan, Henderson, Farabow, Garrett and Dunner, L.L.P. ·
Primary Examiner Bao Thuy L. Nguyen ·
Application Number US8445584
Filing date 05/22/1995
Issue date 10/14/2003
Predicted expiration date 05/20/2008
U.S. Classifications 436/513  · 436/820  · 436/523  · 436/538  · 436/548  · 436/518  · 435/772  · 435/79  · 435/794  · 435/962  · 436/512  ·
International Classifications --
Kind CodeB1
International Classifications 436518 · 436523 · 436538 · 436548 · 436513 · 436512 · 436820 · 435 772 · 435 79 · 435 794 · 435962 ·
Related U.S. Application DataThis application is a continuation application filed May 22, 1995 of parent application U.S. Ser. No. 07/988,007, filed Dec. 9, 1992, now abandoned, which is a continuation application of Ser. No. 07/670,523 filed Mar. 18, 1991, now abandoned, which is a continuation application of Ser. No. 07/434,472 filed Nov. 14, 1989, now abandoned, which is a continuation application of Ser. No. 07/196,526 filed May 20, 1988, now abandoned.
Foreign Priority DE3717401 - 05/23/1987 ·
16 Claims, No Drawings


Abstract

An immunochemical method for the determination of antibodies which are specific for an antigen and are of one of the immunoglobulin classes: A, M, D or E in a fluid, with this fluid being contacted with a solid phase to which the antibodies against this immunoglobulin class, or a fragment of an antibody of this type, are bound, which results in the immunoglobulin of this class being bound to this solid phase, and this solid phase being contacted with the antigen, which carries a labeling means where appropriate, and with a labeled antibody or a labeled fragment of an antibody against the antigen if the antigen is unlabeled and determination, from the amount of labeling means which is bound to the solid phase, of the amount of these antibodies which are specific for an antigen and are one of the immunoglobulin classes, which comprises the solid phase being simultaneously in contact with the fluid containing the antibody which is to be determined and with the antigen, which is labeled where appropriate, there being addition of a substance which prevents immunoglobulin G binding to the solid phase and, where appropriate, antigen binding to immunoglobulin G, and an agent suitable for this purpose, are described.

Independent Claims | See all claims (16)

  1. 1. An immunological method for the detection of an antigen specific antibody comprising one or more of the immunoglobulin classes A, M, D, or E in a fluid, comprising the simultaneous incubation of: a. a solid phase having bonded thereto an antibody specific for said immunoglobulin classes A, M, D, or E; b. a fluid containing an immunoglobulin of classes A, M, D, or E; c. an unlabeled antigen immunologically reactive to the fluid phase immunoglobulin; d. a labeled antibody, immunologically reactive with said unlabeled antigen; and e. a substance which inhibits the binding of immunoglobulin G to the solid phase and which inhibits the binding of said unlabeled antigen to immunoglobulin G.
  2. 9. An immunological method for the detection of an antigen specific antibody comprising one or more of the immunoglobulin classes A, M, D or E in a fluid, comprising the simultaneous incubation of: a. a solid phase having bonded thereto an antibody specific for said immunoglobulin classes A, M, D, or E; b. a fluid containing an immunoglobulin of classes A, M, D, or E; c. a labeled antigen immunologically reactive to the fluid phase immunoglobulin; and d. a substance which inhibits the binding of immunoglobulin G to the solid phase and which inhibits the binding of said labeled antigen to immunoglobulin G.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US4130634* University of Illinois Foundation Molinaro et al. Dec 1978
US4273756* Abbott Laboratories Ling Jun 1981
US4292403* Akzona Incorporated Duermeyer Sep 1981
US4347311* MEDAC Gesellschaft fur Klinishce Spezialpraparate mbH Schmitz Aug 1982
US4434227* Abbott Laboratories Unger Feb 1984
US4477576* Mex Research Associates Deutsch et al. Oct 1984
US4486530* Hybritech Incorporated David et al. Dec 1984
US4663277 Profile Diagnostic Sciences Inc. Wang May 1987
US4962023* Louisiana State University, Agricultural and Mechanical College Todd et al. Oct 1990

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
EP0008473Akzo N.V.Mar 1980
EP0083869INTERNATIONAL INSTITUTE OF CELLULAR AND MOLECULAR PATHOLOGYJul 1983
EP0061167BEHRINGWERKE AKTIENGESELLSCHAFTJul 1985
EP0163312DAIICHI PURE CHEMICALS CO. LTD.Dec 1985
EP0261493SAVYON DIAGNOSTICS LTD.Mar 1988
GB2026691ABBOTT LABFeb 1980
* cited by examiner

Other Publications

H. Schmitz, Detection of IgM Antibodies to Cytomegalovirus Using an Enzyme—Labelled Antigen, 1980, V. 50, p 59-68.*
Duermeyer W. Enzyme Linked Immunosorbent Assay for Detection of Immunoglobulin M Antibodies Against
Wiellaard, F, Diagnosis of Acute Toxoplasmosis by an Enzyme Immunoassay for Specific Immunoglobulin M Antibodies 1983, 17/6, 981-987.*
Sachers, M. et al., J. Virological Methods, 10:99-110 (1985).
Schmitz, H. et al., J. Gen. Virol., 50:59-68, 1980.
Nakane, P.K. et al., “Peroxidase-Labeled Antibody A New Method of Conjugation” J. Histochem. Cytochem.: 22(12); 1084-1091 (1974).
Ishikawa, E. et al., “Enzyme-Labeling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining”, J. Immunoassay, 4(3): 209-327 (1983).
Bergmeyer, Methods of Enzymatic Analysis, Vol. X, Antigens and Antibodies 1, pp. 292-308 (3rd ed. 1986).

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