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US7037716: Packaging systems for human recombinant adenovirus to be used in gene therapy


Filing Information

Inventor(s) Ronald Vogels · Abraham Bout ·
Assignee(s) Crucell Holland B.V. ·
Attorney/Agent(s) TraskBritt ·
Primary Examiner David Guzo ·
Application Number US10396548
Filing date 03/25/2003
Issue date 05/02/2006
Prior Publication Data
Predicted expiration date 12/29/2018
Patent term adjustment 249
U.S. Classifications 435/320.1  · 536/237.2  · 536/241  · 536/231  ·
International Classifications C12N1500  ·
Kind CodeB2
This application is a continuation of application Ser. No. 09/900,062, filed Jul. 6, 2001, now abandoned, which was a divisional of application Ser. No. 09/065,752, filed Apr. 24, 1998, now U.S. Pat. No. 6,670,188, issued Dec. 30, 2003.
2 Claims, 27 Drawings


The invention provides improved methods and products based on adenoviral materials which can advantageously be used in gene therapy. In one aspect, an adenoviral vector is provided having no overlap with a suitable packaging cell line. The suitable packaging cell line is another aspect. The combination excludes the possibility of homologous recombination, thereby excluding the possibility of the formation of replication competent adenovirus. Another aspect embodies an adenovirus based helper construct which by its size is incapable of being encapsidated. This helper virus can be transferred into any suitable host cell making it a packaging cell. Furthermore, a number of useful mutations to adenoviral based materials and combinations of such mutations are disclosed, all of which have in common the safety of the methods and the products, in particular avoiding the production of replication competent adenovirus and/or interference with the immune system.

Independent Claims | See all claims (2)

  1. 1. A recombinant adenovirus wherein the E3-gp19K coding region is replaced by a transgene, wherein any remaining E3 coding regions are intact, which transgene does not comprise a heterologous promoter and which transgene is under transcriptional control of the adenovirus E3 promoter.
  2. 2. A plasmid comprising an adenovirus E3 region wherein the E3-gp19K coding region is replaced by a transgene, wherein the remaining E3 coding regions are intact, which transgene does not comprise a heterologous promoter and which transgene is under transcriptional control of the adenovirus E3 promoter.

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Other Publications

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Referenced By

The current document is not referenced by other documents.

Patent Family

Document NumberAssigneeInventorsIssue/Pub Date
US20050074885 Ronald Vogels et al. Apr 2005
US7037716 Crucell Holland B.V. Ronald Vogels et al. May 2006