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US7112725: Plants having high plant map values


Filing Information

Inventor(s) Karen L Fincher · Stanislaw Flasinski · Jack Q Wilkinson ·
Assignee(s) Monsanto Technology LLC ·
Attorney/Agent(s) Todd M. Rands · Howrey LLP ·
Primary Examiner Dave Trong Nguyen ·
Assistant Examiner Maria Marvich ·
Application Number US10909860
Filing date 08/02/2004
Issue date 09/26/2006
Prior Publication Data
Predicted expiration date 12/15/2020
U.S. Classifications 800/300  · 536/232  · 435/413  · 536/231  · 435/320.1  · 536/236  · 536/241  · 800/278  ·
International Classifications A01H100  · A01H500  · C07H2102  · C07H2104  · C12N1500  · C12N504  ·
Kind CodeB2
Related U.S. Application DataThis application is a continuation of U.S. patent application Ser. No. 10/427,169 filed May 1, 2003 (U.S. Pat. No. 6,919,495 issued July 19. 2005), which is a divisional of U.S. patent application Ser. No. 09/737,626 filed Dec. 15, 2000 (U.S. Pat. No. 6,660,911 issued Dec. 9, 2003), which claimed priority to U.S. Provisional Patent Application Ser. No. 60/171,173 filed Dec. 16, 1999.
17 Claims, 18 Drawings


The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.

Independent Claims | See all claims (17)

  1. 1. Transgenic cotton seed comprising one or more DNA expression constructs, said constructs comprising a hybrid promoter DNA sequence comprising the 34S FMV promoter operably linked to an Arabidopsis EF1α promoter; a structural DNA sequence encoding a glyphosate tolerance protein; and a 3′ non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence; wherein the structural DNA sequence is operably linked to the hybrid promoter and the 3′ non-translated region.
  2. 10. A transgenic cotton plant transformed with a nucleic acid molecule comprising a structural DNA sequences encoding a glyphosate tolerance protein operably linked to a hybrid promoter comprising the 34S FMV promoter operably linked to an Arabidposis EF1a promoter which hybrid promoter drives sufficient expression of the protein in the plant cells to provide vegetative reproductive glyphosate tolerance to the plant, said plant characterized as being capable of having a boll map value greater than or equal to 3 following exposure to glyphosate after the 4 leaf stage.

References Cited

U.S. Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
US5106739 Calgene, Inc. Comai et al. Apr 1992
US5424200 Monsanto Company McPherson et al. Jun 1995
US5633435 Monsanto Company Barry et al. May 1997
US6407316* Rhone-Poulenc AG Company Inc. Holmes et al. Jun 2002
US6660911* Monsanto Technology LLC Fincher et al. Dec 2003

Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
WO199200377Jan 1992
WO199905265Feb 1999
WO199923232May 1999
* cited by examiner

Other Publications

Jones and Snipes, Tolerance of Trangenic Cotton to Topical Applications of Glyphosate, J Cotton Science, 1999, vol. 3, pp. 19-26.*
Pettigrew, W.T., Moisture Deficit Effects on Cotton Lint Yield, Yield Components, and Boll Distribution, 2004, Agronomy Journal, vol. 96, pp. 377-383.*
An Yong-Qiang et al: “Strong constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues.” Plant Journal, vol. 10, No. 1, 1996, pp. 107-121.
K. Datta et al., “Constitutive and Tissue-Specific Differential Expression of the crylA(b) Gene in Transgenic Rice Plants Conferring Resistance to Rice Insect Pest,” Theoretical and Applied Genetics, 97(1-2):20-30 (1998).
Ildikö Vibók et al., “Endosperm Specific Expression of a Gliadin-Actin Hybrid Promoter in Transgenic Rice (Oryza Sativa L.),” Cereal Research Communications, 27(3):241-249 (1999).
Kay et al., “Duplication of CaMV 355 Promoter Sequences Creates a Strong Enhancer for Plant Genes,” Science, 236(4806):1299-1302 (1987).
Omirulleh et al., “Activity of a Chimeric Promoter with the Doubled CaMV 35S Enhancer Element in Protoplast-Derived Cells and Transgenic Plants in Maize,” Plant Molecular Biology, 21(3):415-428 (1993).
Maiti et al., “Promoter/Leader Deletion Analysis and Plant Expression Vectors with the Figwort Mosaic Virus (FMV) Full Length Transcript (FLt) Promoter Containing Single or Double Enhancer Domains,” Transgenic Research. 6(2):143-156 (1997).
* cited by examiner

Referenced By

The current document is not referenced by other documents.