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US7344883: Complementing cell lines


Filing Information

Inventor(s) Ronald Vogels · Menzo Jans Emco Havenga · Majid Mehtali ·
Assignee(s) Crucell Holland B.V. ·
Attorney/Agent(s) TraskBritt ·
Primary Examiner Sumesh Kalishal ·
Assistant Examiner Michael Burkhart ·
Application Number US11165697
Filing date 06/24/2005
Issue date 03/18/2008
Prior Publication Data
Predicted expiration date 11/15/2020
U.S. Classifications 435/325  · 435/455  · 435/235.1  · 435/320.1  · 435/366  ·
International Classifications C12N15861  · C12N508  · C12N510  · C12N700  ·
Kind CodeB2
This application is a continuation of application U.S. Ser. No. 10/002,750, filed Nov. 15, 2001, now U.S. Pat. No. 6,974,695, which is a continuation-in-part of application Ser. No. 09/713,678, filed Nov. 15, 2000, now U.S. Pat. No. 6,492,169, issued Dec. 10, 2002, the contents of all of which are incorporated by this reference.
15 Claims, 38 Drawings


A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6 that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination. The cell lines can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. Also, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, and measles virus.

Independent Claims | See all claims (15)

  1. 1. A packaging cell line capable of complementing a recombinant adenovirus based on a serotype from subgroup B said recombinant adenovirus lacking a functional E1 region, wherein said packaging cell line comprises functional E1A and E1B-21K coding sequences, and wherein said packaging cell line further comprises a functional E1B-55K coding sequence of a serotype from subgroup B, wherein at least one of the E1 coding sequence is driven by a non-adenoviral promoter.
  2. 12. A PER.C6 (ECACC deposit number 96022940) cell line, comprising an Ad35-E1B coding sequence.

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