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US7442159: Selection system

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Filing Information

Inventor(s) Lutz Riechmann · Peter Kristensen · Jean-Luc Jestin · Gregory Paul Winter ·
Assignee(s) Domantis Limited ·
Attorney/Agent(s) Kathleen Williams · Edwards Angell Palmer & Dodge LLP ·
Primary Examiner Jon D. Epperson ·
Assistant Examiner Amber D. Steele ·
Application Number US9710444
Filing date 11/10/2000
Issue date 10/28/2008
Predicted expiration date 05/30/2021
Patent term adjustment 748
U.S. Classifications 506/6  · 435/6  · 506/14  · 506/9  ·
International Classifications C12Q168  · C40B2008  · C40B3004  · C40B4002  ·
Kind CodeB1
Related U.S. Application DataThis is a continuation of International Application GB99/01526, with an international filing date of May 13, 1999, now abandoned. The invention relates to a selection system which permits the selection of polypeptides displayed in a phage display system.
Foreign Priority GB9810223.9 - 05/13/1998 · GB9810228.8 - 05/13/1998 ·
21 Claims, 6 Drawings


Abstract

The present invention concerns a method for the selection of a virus comprising the steps of: (a) providing a virus encoding and displaying a fusion polypeptide, said fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a viral coat protein polypeptide, wherein said virus comprises a cleavable site located within a displayed polypeptide; (b) exposing the virus to a cleaving agent; (c) propagating the virus comprising intact fusion protein.

Independent Claims | See all claims (21)

  1. 1. A method for the selection of a bacteriophage comprising the steps of: (a) providing a plurality of bacteriophage encoding and displaying a fusion polypeptide, said displayed fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a bacteriophage coat wherein said plurality of bacteriophage comprise a sequence specific protease cleavable site located within the displayed polypeptide and which site is protected by folding of the displayed fusion polypeptide and is either absent from the bacteriophage other than the site specific insertion, or inaccessible to cleavage, or present only in bacteriophage proteins not required after bacteriophage assembly to mediate infection and wherein cleavage of said sequence specific protease cleavable site impairs infection by a said bacteriophage; (b) exposing the bacteriophage to a protease that recognizes said sequence specific protease cleavable site, wherein said protease only cleaves said sequence specific protease cleavable site if said displayed fusion polypeptide is not properly folded, such that said exposing selects against bacteriophage displaying fusion polypeptide that is not properly folded; and (c) propagating a bacteriophage comprising intact displayed fusion polypeptide.

References Cited

U.S. Patent Documents

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Foreign Patent Documents

Document NumberAssigneesInventorsIssue/Pub Date
WO199005144May 1990
WO199014430Nov 1990
WO199201047Jan 1992
WO199220791Nov 1992
WO199311236Jun 1993
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Referenced By

The current document is not referenced by other documents.