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US7851188: Fermentive production of four carbon alcohols

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Filing Information

Inventor(s) Gail K. Donaldson · Andrew C. Eliot · Dennis Flint · Lori Ann Maggio-Hall · Vasantha Nagarajan ·
Assignee(s) Butamax(TM) Advanced Biofuels LLC ·
Attorney/Agent(s) Christine M. Lhulier ·
Primary Examiner Christian L Fronda ·
Application Number US11586315
Filing date 10/25/2006
Issue date 12/14/2010
Prior Publication Data
Predicted expiration date 06/01/2027
Patent term adjustment 219
U.S. Classifications 435/160  · 435/254.22  · 435/252.3  · 435/183  · 435/320.1  · 435/157  · 435/691  ·
International Classifications C07H2104  · C12N100  · C12N120  · C12N1500  · C12N900  · C12P2106  · C12P704  · C12P716  ·
Kind CodeB2
Related U.S. Application DataCROSS-REFERENCE TO RELATED APPLICATION
This application claims priority under 35 U.S.C. §119 from U.S. Provisional Application Ser. No. 60/730,290, filed Oct. 26, 2005.
37 Claims, 1 Drawings


Abstract

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

Independent Claims | See all claims (37)

  1. 1. A recombinant microbial host cell comprising heterologous DNA molecules encoding polypeptides that catalyze substrate to product conversions for each step below: i) pyruvate to acetolactate; ii) acetolactate to 2,3-dihydroxyisovalerate; iii) 2,3-dihydroxyisovalerate to α-ketoisovalerate; and iv) α-ketoisovalerate to isobutyraldehyde; wherein said microbial host cell produces isobutanol; and wherein a) the polypeptide that catalyzes a substrate to product conversion of pyruvate to acetolactate is acetolactate synthase having the EC number 2.2.1.6; b) the polypeptide that catalyzes a substrate to product conversion of acetolactate to 2,3-dihydroxyisovalerate is acetohydroxy acid isomeroreductase having the EC number 1.1.1.86; c) the polypeptide that catalyzes a substrate to product conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate is acetohydroxy acid dehydratase having the EC number 4.2.1.9; d) the polypeptide that catalyzes a substrate to product conversion of α-ketoisovalerate to isobutyraldehyde is branched-chain α-keto acid decarboxylase having the EC number 4.1.1.72.

References Cited

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Foreign Patent Documents

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Other Publications

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* cited by examiner

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